| Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. | |
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MedLine Citation:
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PMID: 7748494 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mercurated DNA was synthesized in vitro by substituting Hg-dCTP or Hg-dUMP for dCTP in one strand of M13mp8DNA in a DNA polymerase I reaction. Restriction enzymes, including Sma I, Pst I, Bam HI, Hind III, and Hinc II, were completely inactive when their recognition sites were fully substituted with Hg-dCMP, while Hg-dUMP containing DNA was hydroxlyzed to some extent. Under conditions favoring star activities, or when DNA was substituted with a low level of mercury-nucleotide, DNA was cleaved by restriction enzymes. Susceptibility to degrading and synthesizing enzymes and insensitivity to restriction endonucleases of fully mercurated DNA makes mercuration an attractive molecular "tag" for in vitro manipulation and selective isolation of Hg-DNA. |
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Authors:
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G Banfalvi; N Sarkar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: DNA and cell biology Volume: 14 ISSN: 1044-5498 ISO Abbreviation: DNA Cell Biol. Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-06-22 Completed Date: 1995-06-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9004522 Medline TA: DNA Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 445-50 Citation Subset: IM |
Affiliation:
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Department I, Semmelweis University Medical School, Budapest, Hungary. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA / chemistry* DNA Restriction Enzymes / pharmacology* Electrophoresis, Polyacrylamide Gel Escherichia coli / genetics Mercury / chemistry* Molecular Sequence Data |
| Chemical | |
Reg. No./Substance:
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7439-97-6/Mercury; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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