Document Detail

Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases.
MedLine Citation:
PMID:  7748494     Owner:  NLM     Status:  MEDLINE    
Mercurated DNA was synthesized in vitro by substituting Hg-dCTP or Hg-dUMP for dCTP in one strand of M13mp8DNA in a DNA polymerase I reaction. Restriction enzymes, including Sma I, Pst I, Bam HI, Hind III, and Hinc II, were completely inactive when their recognition sites were fully substituted with Hg-dCMP, while Hg-dUMP containing DNA was hydroxlyzed to some extent. Under conditions favoring star activities, or when DNA was substituted with a low level of mercury-nucleotide, DNA was cleaved by restriction enzymes. Susceptibility to degrading and synthesizing enzymes and insensitivity to restriction endonucleases of fully mercurated DNA makes mercuration an attractive molecular "tag" for in vitro manipulation and selective isolation of Hg-DNA.
G Banfalvi; N Sarkar
Related Documents :
291964 - Mechanism for circularization of linear dnas: circular parvovirus mvm dna is formed by ...
18511464 - A recb-family nuclease motif in the type i restriction endonuclease ecor124i.
350714 - Cloning of restriction and modification genes in e. coli: the hbaii system from haemoph...
7840534 - Human mitochondrial dna variation in lithuania.
17541164 - Anchoring cationic amphiphiles for nucleotide delivery: significance of dna release fro...
23529764 - Dna methylation of the p66shc promoter is decreased in placental tissue from women deli...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  DNA and cell biology     Volume:  14     ISSN:  1044-5498     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  445-50     Citation Subset:  IM    
Department I, Semmelweis University Medical School, Budapest, Hungary.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
DNA / chemistry*
DNA Restriction Enzymes / pharmacology*
Electrophoresis, Polyacrylamide Gel
Escherichia coli / genetics
Mercury / chemistry*
Molecular Sequence Data
Reg. No./Substance:
7439-97-6/Mercury; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Molecular cloning and cellular localization of a furin-like prohormone convertase from the atrial gl...
Next Document:  Characterization of placental transcriptional activation of the human gene for P450scc.