Document Detail

Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases.
MedLine Citation:
PMID:  7748494     Owner:  NLM     Status:  MEDLINE    
Mercurated DNA was synthesized in vitro by substituting Hg-dCTP or Hg-dUMP for dCTP in one strand of M13mp8DNA in a DNA polymerase I reaction. Restriction enzymes, including Sma I, Pst I, Bam HI, Hind III, and Hinc II, were completely inactive when their recognition sites were fully substituted with Hg-dCMP, while Hg-dUMP containing DNA was hydroxlyzed to some extent. Under conditions favoring star activities, or when DNA was substituted with a low level of mercury-nucleotide, DNA was cleaved by restriction enzymes. Susceptibility to degrading and synthesizing enzymes and insensitivity to restriction endonucleases of fully mercurated DNA makes mercuration an attractive molecular "tag" for in vitro manipulation and selective isolation of Hg-DNA.
G Banfalvi; N Sarkar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  DNA and cell biology     Volume:  14     ISSN:  1044-5498     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  445-50     Citation Subset:  IM    
Department I, Semmelweis University Medical School, Budapest, Hungary.
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MeSH Terms
Base Sequence
DNA / chemistry*
DNA Restriction Enzymes / pharmacology*
Electrophoresis, Polyacrylamide Gel
Escherichia coli / genetics
Mercury / chemistry*
Molecular Sequence Data
Reg. No./Substance:
7439-97-6/Mercury; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

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