| Effect of medium exchange rate on colony growth of the human tumor cell line MDA-231 cloned within the perfused capillary cloning system. | |
| | |
MedLine Citation:
|
PMID: 8073229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The conventional human tumor stem cell assay is limited by the lack of flexibility for drug scheduling with single agents and its inability to test drug combinations. Recently, we described the cloning of tumor cell lines within porous glass capillary tubes which allow free exchange of substances. The present study describes the influence of various perfusion modalities on the colony growth of the human tumor cell line MDA-231 cloned within the perfused capillary cloning system (PCCS). Colony growth of tumor cells within the PCCS is strongly dependent on perfusion tube volume, flow rate and duration of perfusion with growth medium. Best colony growth was achieved using a perfusion tube volume of 12 ml resulting in a cloning efficiency of 36.3%. Continuous perfusion with fresh medium did not improve the cloning efficiency; in fact, colony growth was hampered compared to colony growth within unperfused porous capillaries. However, cloning efficiency was acceptable when continuous perfusion was started at day 6 (26.4%) instead of day 0 (17.2%), or when a short perfusion with high volume (12 ml/h) was discontinued after 1 h at day 0. In contrast to the conventional capillary cloning system the PCCS has the potential for investigating secretion and kinetics of tumor-specific factors and the effect of growth-stimulating or growth-inhibiting drugs. |
| | |
Authors:
|
H Weisser; J G Witzel; B Lathan |
Related Documents
:
|
21880559 - Knock-down of sox11 induces autotaxin-dependent increase in proliferation in vitro and... 22048879 - Increased tryptophan transport in epileptogenic dysembryoplastic neuroepithelial tumors. 23090259 - Tumor dormancy and the neuroendocrine system: an undisclosed connection? 22013169 - Evaluation of brain tumor vessels specific contrast agents for glioblastoma imaging. 22192169 - Curcumin promotes differentiation of glioma-initiating cells by inducing autophagy. 21347689 - Identification and functional characterization of glioma-specific promoters and their a... 6677839 - A merkel cell tumor of the eyelid. 17631279 - Mechanism of acridine-based telomerase inhibition and telomere shortening. 1810429 - Estradiol induced changes in tumor growth and steroid receptor content in a heterotrans... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine Volume: 15 ISSN: 1010-4283 ISO Abbreviation: Tumour Biol. Publication Date: 1994 |
Date Detail:
|
Created Date: 1994-09-29 Completed Date: 1994-09-29 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8409922 Medline TA: Tumour Biol Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 153-9 Citation Subset: IM |
Affiliation:
|
Institute of Clinical Chemistry and Laboratory Medicine, University Clinic Bergmannsheil, Bochum, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Breast Neoplasms Capillarity Cell Division* Clone Cells Culture Media Culture Techniques / instrumentation*, methods Female Humans Perfusion / methods Tumor Cells, Cultured / cytology* Tumor Stem Cell Assay / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Electrical potential measurements in human breast cancer and benign lesions.
Next Document: Smoking cessation: importance for the patient and role of the practitioner