Document Detail


Effect of medium exchange rate on colony growth of the human tumor cell line MDA-231 cloned within the perfused capillary cloning system.
MedLine Citation:
PMID:  8073229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The conventional human tumor stem cell assay is limited by the lack of flexibility for drug scheduling with single agents and its inability to test drug combinations. Recently, we described the cloning of tumor cell lines within porous glass capillary tubes which allow free exchange of substances. The present study describes the influence of various perfusion modalities on the colony growth of the human tumor cell line MDA-231 cloned within the perfused capillary cloning system (PCCS). Colony growth of tumor cells within the PCCS is strongly dependent on perfusion tube volume, flow rate and duration of perfusion with growth medium. Best colony growth was achieved using a perfusion tube volume of 12 ml resulting in a cloning efficiency of 36.3%. Continuous perfusion with fresh medium did not improve the cloning efficiency; in fact, colony growth was hampered compared to colony growth within unperfused porous capillaries. However, cloning efficiency was acceptable when continuous perfusion was started at day 6 (26.4%) instead of day 0 (17.2%), or when a short perfusion with high volume (12 ml/h) was discontinued after 1 h at day 0. In contrast to the conventional capillary cloning system the PCCS has the potential for investigating secretion and kinetics of tumor-specific factors and the effect of growth-stimulating or growth-inhibiting drugs.
Authors:
H Weisser; J G Witzel; B Lathan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  15     ISSN:  1010-4283     ISO Abbreviation:  Tumour Biol.     Publication Date:  1994  
Date Detail:
Created Date:  1994-09-29     Completed Date:  1994-09-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  153-9     Citation Subset:  IM    
Affiliation:
Institute of Clinical Chemistry and Laboratory Medicine, University Clinic Bergmannsheil, Bochum, Germany.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms
Capillarity
Cell Division*
Clone Cells
Culture Media
Culture Techniques / instrumentation*,  methods
Female
Humans
Perfusion / methods
Tumor Cells, Cultured / cytology*
Tumor Stem Cell Assay / instrumentation*,  methods
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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