Document Detail

Effect of media, additives, and incubation conditions on the recovery of high pressure and heat-injured Clostridium botulinum spores.
MedLine Citation:
PMID:  20510779     Owner:  NLM     Status:  MEDLINE    
The effect of additives and post-treatment incubation conditions on the recovery of high pressure and heat-injured (i.e., processed at 620 MPa and 95 and 100 degrees C for 5 min) spores of Clostridium botulinum strains, 62-A (proteolytic type A) and 17-B (nonproteolytic type B) was studied. High pressure and heat-injured spores were inoculated into TPGY (Trypticase-Peptone-Glucose-Yeast extract) anaerobic broth media containing additives (lysozyme, L-alanine, L-aspartic acid, dipicolonic acid, sodium bicarbonate, and sodium lactate) at various concentrations (0-10 microg/ml) individually or in combination. The spore counts of high pressure and heat-injured 62-A and 17-B recovered from TPGY broth containing lysozyme (10 microg/ml) incubated for 4 months versus that recovered from peptone-yeast extract-glucose-starch (PYGS) plating agar containing lysozyme (10 microg/ml) incubated under anaerobic conditions for 5 days were also compared. None of the additives either individually or in combination in TPGY broth improved recovery of injured spore enumeration compared to processed controls without additives. Addition of lysozyme at concentrations of 5 and 10 microg/ml in TPGY broth improved initial recovery of injured spores of 17-B during the first 4 days of incubation but did not result in additional recovery at the end of the 4 month incubation compared to the processed control without lysozyme. Adding lysozyme at a concentration of 10 microg/ml to PYGS plating agar resulted in no effect on the recovery of high pressure and heat-injured 62-A and 17-B spores. The recovery counts of high pressure and heat-injured spores of 62-A and 17-B were lower (i.e., <1.0 log units) with PYGS plating agar compared to the MPN method using TPGY broth as the growth medium.
N R Reddy; R C Tetzloff; G E Skinner
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Publication Detail:
Type:  Journal Article     Date:  2010-02-19
Journal Detail:
Title:  Food microbiology     Volume:  27     ISSN:  1095-9998     ISO Abbreviation:  Food Microbiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8601127     Medline TA:  Food Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  613-7     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ltd.
National Center for Food Safety and Technology, US Food and Drug Administration, 6502 S. Archer Road, Summit-Argo, IL 60501, USA.
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MeSH Terms
Bacteriological Techniques* / methods
Clostridium botulinum / chemistry,  drug effects,  growth & development
Culture Media / pharmacology*
Food Additives / pharmacology*
Hot Temperature
Microbial Viability / drug effects
Spores, Bacterial / chemistry,  drug effects,  growth & development*
Reg. No./Substance:
0/Culture Media; 0/Food Additives

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