| Effect of mechanical factors on the function of engineered human blood microvessels in microfluidic collagen gels. | |
| | |
MedLine Citation:
|
PMID: 20537705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This work examines how mechanical signals affect the barrier function and stability of engineered human microvessels in microfluidic type I collagen gels. Constructs that were exposed to chronic low flow displayed high permeabilities to bovine serum albumin and 10 kDa dextran, numerous focal leaks, low size selectivity, and short lifespan of less than one week. Higher flows promoted barrier function and increased longevity; at the highest flows, the barrier function rivaled that observed in vivo, and all vessels survived to day 14. By studying the physiology of microvessels of different geometries, we established that shear stress and transmural pressure were the dominant mechanical signals that regulated barrier function and vascular stability, respectively. In microvessels that were exposed to high flow, elevation of intracellular cyclic AMP further increased the selectivity of the barrier and strongly suppressed cell proliferation. Computational models that incorporated stress dependence successfully predicted vascular phenotype. Our results indicate that the mechanical microenvironment plays a major role in the functionality and stability of engineered human microvessels in microfluidic collagen gels. |
| | |
Authors:
|
Gavrielle M Price; Keith H K Wong; James G Truslow; Alexander D Leung; Chitrangada Acharya; Joe Tien |
Related Documents
:
|
10779695 - The blood-nerve barrier: enzymes, transporters and receptors--a comparison with the blo... 4022265 - Aluminum alters the permeability of the blood-brain barrier to some non-peptides. 2456895 - The effects of 3-isobutyl-methyl-xanthine on experimentally induced ocular inflammation... 22573015 - Cerebral autoregulation and acute ischemic stroke. 16045175 - Case with bromine exposure leading to respiratory insufficiency. 9575935 - Vasomotor responses in chronically hyperperfused and hypoperfused rat mesenteric arteries. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-05-26 |
Journal Detail:
|
Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-06-11 Completed Date: 2010-09-20 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
|
Languages: eng Pagination: 6182-9 Citation Subset: IM |
Copyright Information:
|
2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Proliferation
/
drug effects Collagen / pharmacology* Computer Simulation Cyclic AMP / metabolism Gels / pharmacology* Hemorheology / drug effects Humans Microfluidics / methods* Microvessels / drug effects*, growth & development, physiology* Phenotype Pressure Stress, Mechanical* Time Factors Tissue Engineering / methods* |
| Grant Support | |
ID/Acronym/Agency:
|
EB005792/EB/NIBIB NIH HHS; HL092335/HL/NHLBI NIH HHS; R01 EB005792-04/EB/NIBIB NIH HHS; R21 HL092335-01A1/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Gels; 60-92-4/Cyclic AMP; 9007-34-5/Collagen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulating orientation and phenotype of primary vascular smooth muscle cells by biodegradable films ...
Next Document: Impact of cytogenetics in patients with relapsed or refractory multiple myeloma treated with bortezo...