Document Detail


Effect of maternal dexamethasone treatment and ambient temperature on prolactin receptor abundance in Brown adipose and hepatic tissue in the foetus and new-born lamb.
MedLine Citation:
PMID:  10520135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the influence of maternal dexamethasone treatment and ambient temperature on prolactin receptor (PRLR) abundance in brown adipose tissue (BAT) and hepatic tissue from foetuses and 6-h-old lambs delivered by caesarean section. Lambs were either delivered into a warm (30 degrees C; WD) or cool (15 degrees C; CD) ambient temperature at 140 days gestation, 2 days after dexamethasone treatment, or at 146 days gestation for controls. Uncoupling protein-1 (UCP1) content of BAT was higher in dexamethasone-treated groups compared to controls. A range of tissue-specific PRLR isoforms was detected. For the long form of PRLR in BAT these isoforms had molecular weights of 66, 54, 34 and 19 kD compared with 88, 76, 66, 58, 54 and 48 kD in liver. In BAT, isoforms of the short form of PRLR had molecular weights of 66, 62, 54, 48, 33 and 31 kD compared with 82, 66, 56, 54, 48, 40 and 33 kD in liver. Dexamethasone treatment in CD lambs resulted in higher abundance of the 54 kD isoform of the short form of PRLR in liver, whilst in BAT dexamethasone resulted in a greater abundance of the 48 kD isoform of the short form, and lower abundance of the 66 kD isoform of the long form of PRLR, compared to controls. A negative correlation (r2 = 0.52) was observed between abundance of 66 kD isoform for the long form of PRLR and UCP1, compared with positive correlations (r2 = 0.58-0.60) for the abundance of the 54 and 48 kD isoforms for the short form of PRLR and UCP1. In conclusion, maternal dexamethasone treatment 1 week before term alters the abundance of PRLR isoforms in a tissue-specific manner. This response is dependent on ambient temperature after birth and may provide a critical endocrine signal for maximising non-shivering thermogenesis.
Authors:
J Bispham; L Heasman; L Clarke; P M Ingleton; T Stephenson; M E Symonds
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroendocrinology     Volume:  11     ISSN:  0953-8194     ISO Abbreviation:  J. Neuroendocrinol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-23     Completed Date:  1999-11-23     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  8913461     Medline TA:  J Neuroendocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  849-56     Citation Subset:  IM    
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham, UK.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue, Brown / drug effects,  embryology,  metabolism*
Animals
Animals, Newborn
Cell Membrane / metabolism
Cesarean Section
Dexamethasone / pharmacology*
Female
Gestational Age
Liver / drug effects,  embryology,  metabolism*
Mitochondria / metabolism
Pregnancy
Prenatal Exposure Delayed Effects*
Protein Isoforms / drug effects,  metabolism
Receptors, Prolactin / drug effects,  metabolism*
Sheep
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Protein Isoforms; 0/Receptors, Prolactin; 50-02-2/Dexamethasone

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