Document Detail

Effect of mannose on Salmonella typhimurium-mediated loss of mucosal epithelial integrity in cultured chick intestinal segments.
MedLine Citation:
PMID:  7980275     Owner:  NLM     Status:  MEDLINE    
The effect of incubating Salmonella typhimurium and S. typhimurium cell-free extracts with isolated intestinal segments from 1-day-old chicks was evaluated using scanning electron microscopy. Incubation of segments with intact bacteria or with cell-free extract resulted in the loss of mucosal epithelial integrity after as little as 30 min incubation. Loss of mucosal epithelial integrity was evidenced by the complete shedding of the epithelium. The addition of 2.5% D-mannose to the incubation medium inhibited the loss of epithelial cells, whether intestinal segments were incubated with intact bacteria or with cell-free extract. These results indicate that S. typhimurium exerts a D-mannose-sensitive cytotoxic effect on the mucosal epithelium of isolated intestinal segments and that the cytotoxic effector is present in cell-free extracts of the bacteria.
R E Droleskey; B A Oyofo; B M Hargis; D E Corrier; J R DeLoach
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Avian diseases     Volume:  38     ISSN:  0005-2086     ISO Abbreviation:  Avian Dis.     Publication Date:    1994 Apr-Jun
Date Detail:
Created Date:  1994-11-29     Completed Date:  1994-11-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370617     Medline TA:  Avian Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  275-81     Citation Subset:  IM    
USDA/ARS Food Animal Protection Research Laboratory, College Station, Texas 77845.
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MeSH Terms
Cell-Free System
Epithelium / drug effects,  microbiology,  ultrastructure
Intestinal Mucosa / drug effects,  microbiology,  ultrastructure*
Intestine, Small
Mannose / pharmacology*
Microscopy, Electron, Scanning
Organ Culture Techniques
Salmonella typhimurium / drug effects,  pathogenicity*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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