| Effect of mannose on Salmonella typhimurium-mediated loss of mucosal epithelial integrity in cultured chick intestinal segments. | |
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MedLine Citation:
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PMID: 7980275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of incubating Salmonella typhimurium and S. typhimurium cell-free extracts with isolated intestinal segments from 1-day-old chicks was evaluated using scanning electron microscopy. Incubation of segments with intact bacteria or with cell-free extract resulted in the loss of mucosal epithelial integrity after as little as 30 min incubation. Loss of mucosal epithelial integrity was evidenced by the complete shedding of the epithelium. The addition of 2.5% D-mannose to the incubation medium inhibited the loss of epithelial cells, whether intestinal segments were incubated with intact bacteria or with cell-free extract. These results indicate that S. typhimurium exerts a D-mannose-sensitive cytotoxic effect on the mucosal epithelium of isolated intestinal segments and that the cytotoxic effector is present in cell-free extracts of the bacteria. |
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Authors:
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R E Droleskey; B A Oyofo; B M Hargis; D E Corrier; J R DeLoach |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Avian diseases Volume: 38 ISSN: 0005-2086 ISO Abbreviation: Avian Dis. Publication Date: 1994 Apr-Jun |
Date Detail:
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Created Date: 1994-11-29 Completed Date: 1994-11-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370617 Medline TA: Avian Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 275-81 Citation Subset: IM |
Affiliation:
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USDA/ARS Food Animal Protection Research Laboratory, College Station, Texas 77845. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell-Free System Chickens Epithelium / drug effects, microbiology, ultrastructure Intestinal Mucosa / drug effects, microbiology, ultrastructure* Intestine, Small Mannose / pharmacology* Microscopy, Electron, Scanning Organ Culture Techniques Salmonella typhimurium / drug effects, pathogenicity* |
| Chemical | |
Reg. No./Substance:
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31103-86-3/Mannose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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