Document Detail


Effect of maitotoxin on sea urchin egg fertilization and on Ca2+ permeabilities of eggs and intracellular stores.
MedLine Citation:
PMID:  1794068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maitotoxin (MTX), a potent marine toxin involved in ciguatera poisoning, inhibited sea urchin egg fertilization in a dose-dependent manner with an IC50 of 7.5 x 10(-3) MU (mouse-unit)/ml. It did not affect male gametes fertilizing capabilities but provoked exocytosis in female gametes. It induced a K+ loss simultaneously with a Na+ entry into unfertilized eggs and increased the Ca2+ influx at higher concentrations. On isolated cortex preparations, high concentrations of MTX reduced the rate of ATP-dependent Ca2+ accumulation into reticulum compartments and caused a leakage of Ca2+ from a preparation pre-loaded with 45Ca2+. Verapamil (10(-4) M) similarly blocked the increase of egg permeability to Ca2+ and the effect on Ca2+ sequestering into intracellular compartment, induced by MTX. Ion transport perturbations which evolved relatively slowly are probably not the direct cause of fertilization inhibition which could be related to a modification of the plasma membrane of the female gametes by this hydrophilic toxin.
Authors:
D Pesando; J P Girard; M Durand-Clément; P Payan; S Puiseux-Dao
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  72     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  1991  
Date Detail:
Created Date:  1992-04-03     Completed Date:  1992-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  269-73     Citation Subset:  IM    
Affiliation:
Unité 303 INSERM, Villefranche-sur-Mer, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Cell Membrane Permeability
Dose-Response Relationship, Drug
Endoplasmic Reticulum / drug effects,  metabolism
Fertilization / drug effects*
Kinetics
Male
Marine Toxins / isolation & purification,  pharmacology*
Ovum / drug effects,  physiology*
Oxocins*
Potassium / metabolism
Sea Urchins
Sodium / metabolism
Spermatozoa / drug effects,  physiology*
Verapamil / pharmacology
Chemical
Reg. No./Substance:
0/Marine Toxins; 0/Oxocins; 52-53-9/Verapamil; 59392-53-9/maitotoxin; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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