Document Detail

Effect of magnesium on secretion of platelet-derived growth factor by cultured human arterial smooth muscle cells.
MedLine Citation:
PMID:  8878004     Owner:  NLM     Status:  MEDLINE    
Two conditioned media were prepared by culturing human umbilical artery smooth muscle cells (SMC) in 75 cm2 flasks with minimum essential medium (MEM) under magnesium (Mg) sufficient (900 microM) or deficient (100 microM) conditions for 72 h ([900]- and [100]-MEM), respectively. A third conditioned medium was obtained by adjusting the Mg concentration of half of the [100]-MEM to 900 microM ([100-900]-MEM). SMC in 12-well plates were incubated in one of the three conditioned media and the growth rates of SMC were determined by [3H]-thymidine incorporation and cell counting. The growth rate in [100-900]-MEM was significantly higher than in [900]- and [100]-MEM. When platelet derived growth factor (PDGF) was neutralized by the addition of a mixture of anti-PDGF-AA and -BB antibodies, [3H]-thymidine incorporation in [100-900]-MEM decreased by 23.3 per cent, but only by 7.0 per cent in [900]-MEM. The quantity of PDGF in the Mg-deficient media was greater than in the magnesium-sufficient media at all indicated times, as shown by radioimmunoassay for PDGF-BB or -AB. These results indicate that Mg deficiency increases the secretion of PDGF by SMC.
S Yokoyama; T L Smith; K K Kawano; F A Kummerow
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Magnesium research : official organ of the International Society for the Development of Research on Magnesium     Volume:  9     ISSN:  0953-1424     ISO Abbreviation:  Magnes Res     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1997-01-16     Completed Date:  1997-01-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8900948     Medline TA:  Magnes Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  93-9     Citation Subset:  IM    
Burnsides Research Laboratory, University of Illinois, Urbana 61801, USA.
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MeSH Terms
Antibodies / pharmacology
Cell Division / drug effects
Cells, Cultured
Culture Media, Conditioned
Magnesium / pharmacology
Magnesium Deficiency / metabolism*
Muscle, Smooth, Vascular / cytology,  drug effects,  metabolism*
Platelet-Derived Growth Factor / drug effects,  immunology,  secretion*
Umbilical Arteries
Reg. No./Substance:
0/Antibodies; 0/Culture Media, Conditioned; 0/Platelet-Derived Growth Factor; 7439-95-4/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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