Document Detail


Effect of magnesium deficiency on delta 6 desaturase activity and fatty acid composition of rat liver microsomes.
MedLine Citation:
PMID:  2555646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experimental Mg2+ deficiency was induced in a group of rats by feeding them a Mg2+-deficient diet for 23 days. They were pair-fed to compare with a control group of rats fed a Mg2+-sufficient diet. In the Mg2+-deficient group the plasma total cholesterol and triglyceride levels were increased while HDL-cholesterol was decreased. In the Mg2+-deficient group the plasma level of thiobarbituric acid reacting substances (TBARS) used as a measure for lipid peroxidation was increased. The increase was attributed to the increased cytosolic Ca2+ in Mg2+-deficiency which can cause: 1) increase of hydro and endoperoxide levels as a consequence of the increase of arachidonic acid release and eicosanoid synthesis in Mg2+-deficiency, and 2) inhibition of the mitochondrial respiratory activity and activation of Ca2+-dependent proteases which may activate the conversion of xanthine dehydrogenase to xanthine oxidase which generates active O2 species. In the Mg2+-deficient group, the fatty acid composition of the liver microsomes indicated a slower rate of conversion of linoleic acid to arachidonic acid which was consistent with the decrease of delta 6 desaturase activity in liver microsomes of Mg2+-deficient rats as measured in vitro. The decrease of delta 6 desaturase activity was attributed to the lower concentration of actual enzyme molecules as a result of the decreased rate of protein synthesis in Mg2+-deficiency. The possible effects of the increased catecholamine release in Mg2+-deficiency are discussed.
Authors:
M M Mahfouz; F A Kummerow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  24     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-12-29     Completed Date:  1989-12-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  727-32     Citation Subset:  IM    
Affiliation:
Burnsides Research Laboratory, University of Illinois, Urbana 61801.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Arachidonic Acid
Arachidonic Acids / metabolism
Cholesterol / blood
Cholesterol, HDL / blood
Coenzyme A / metabolism
Cyclic AMP / metabolism
Fatty Acid Desaturases / metabolism*
Fatty Acids / metabolism*
Fatty Acids, Nonesterified / metabolism
Hydroxymethylglutaryl CoA Reductases / metabolism
Linoleoyl-CoA Desaturase
Lipid Peroxides / blood
Magnesium / pharmacology
Magnesium Deficiency / enzymology*
Male
Microsomes, Liver / enzymology*
Rats
Rats, Inbred Strains
Thiobarbiturates
Triglycerides / blood
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Cholesterol, HDL; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Lipid Peroxides; 0/Thiobarbiturates; 0/Triglycerides; 504-17-6/thiobarbituric acid; 506-32-1/Arachidonic Acid; 56-65-5/Adenosine Triphosphate; 57-88-5/Cholesterol; 60-92-4/Cyclic AMP; 7439-95-4/Magnesium; 85-61-0/Coenzyme A; EC 1.1.1.-/Hydroxymethylglutaryl CoA Reductases; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.19.3/Linoleoyl-CoA Desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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