Document Detail


Effect of macromolecules from oviductal conditioned medium on bovine sperm motion and capacitation.
MedLine Citation:
PMID:  7819461     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of macromolecules from oviductal conditioned medium (CM) on sperm motility and capacitation was studied. Sperm pooled from three bulls was incubated in either luteal isthmic CM, luteal ampullary CM, estrual isthmic CM, estrual ampullary CM, or control medium (no CM) for 4 h. Sperm capacitation and motility were assessed at 10 min and 4 h. Estrual isthmic CM capacitated significantly more spermatozoa at 4 h than estrual ampullary CM or control medium. CM also affected lateral head movement (ALH) and beat cross-frequency (BCF) of sperm. In a second experiment, the glycosaminoglycan (GAG) content of the different types of oviductal CM was quantified. Estrual isthmic CM contained more GAG than estrual ampullary CM. Among luteal samples, no difference in GAG concentration between the isthmic and ampullary CM was found. Heat treatment (100 degrees C) of oviductal CM before coincubation with sperm significantly reduced, but did not eliminate, the capacitating ability. Because heat treatment denatures proteins and decreases the capacitating ability of certain GAG, we concluded that the capacitating effect of estrual isthmic CM may be associated with proteins, GAG, and proteoglycans in the CM. Isthmic secretions may play a major role during in vivo sperm capacitation, given that bovine spermatozoa may reside in the oviduct isthmus for up to 18 h before fertilization.
Authors:
S H Anderson; G J Killian
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  51     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-02-13     Completed Date:  1995-02-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  795-9     Citation Subset:  IM    
Affiliation:
Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle*
Culture Media, Conditioned*
Estrus / physiology
Fallopian Tubes / metabolism*
Female
Glycosaminoglycans / analysis,  pharmacology
Hot Temperature
Male
Protein Denaturation
Proteins / analysis,  pharmacology
Proteoglycans / pharmacology
Sperm Capacitation* / drug effects
Sperm Motility* / drug effects
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Glycosaminoglycans; 0/Proteins; 0/Proteoglycans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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