| Effect of luteinizing hormone and tumour necrosis factor-alpha on VEGF secretion by cultured porcine endometrial stromal cells. | |
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MedLine Citation:
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PMID: 20586953 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To cope with rising demands for increased blood supply during pregnancy, the vasculature of the uterus undergoes several adaptive changes, including increased permeability, angiogenesis and vasodilatation. Although it is clear that vascular endothelial growth factor (VEGF) plays a paramount role in achieving these adaptations, little is known about regulation of VEGF expression in endometrium during pregnancy. Thus, we have investigated whether luteinizing hormone (LH) and tumour necrosis factor-alpha (TNFalpha) may affect VEGF secretion by stromal cells during early pregnancy in pigs. Real-time reverse transcription/polymerase chain reaction (RT/PCR) of VEGF120 and VEGF164 gene expression revealed significantly higher levels of VEGF164 mRNA in cultured stromal cells (p < 0.0001). The LH-stimulated secretion of VEGF was detected after 24 and 48 h of treatment when doses 50 and 100 ng/ml were used (p < 0.05 and p < 0.01, respectively). The TNFalpha-induced secretion of VEGF by stromal cells was detected only after 24-h treatment with the highest dose used in the experiment (50 ng/ml; p < 0.05). Although the influence of LH on VEGF secretion was more visible compared with TNFalpha, both factors may be considered as potential modulators of adaptive changes in uterine vasculature occurring during pregnancy in the pig. |
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Authors:
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M M Kaczmarek; A Blitek; D Schams; A J Ziecik |
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9509983 - Transforming growth factor beta and the endometrium. 7758833 - Suppressed dna repair capacity of peripheral lymphocytes in pregnant women. 8187923 - Heterotopic ovarian pregnancy after clomiphene ovulation induction. |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproduction in domestic animals = Zuchthygiene Volume: 45 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-30 Completed Date: 2010-09-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 481-6 Citation Subset: IM |
Affiliation:
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Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland. moka@pan.olsztyn.pl |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Endometrium / cytology* Female Gene Expression Gestational Age Luteinizing Hormone / pharmacology* Pregnancy RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Stromal Cells / chemistry, drug effects, secretion* Swine* Tumor Necrosis Factor-alpha / pharmacology* Vascular Endothelial Growth Factor A / genetics, secretion* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 0/Vascular Endothelial Growth Factor A; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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