Document Detail

Effect of low-level laser irradiation on osteoblast proliferation and bone formation.
MedLine Citation:
PMID:  22217992     Owner:  NLM     Status:  MEDLINE    
Applications of laser therapy in biostimulation and healing injured tissues are widely described in medical literature. The present study focuses on the effects of laser irradiation on the growth rate and differentiation of human osteoblast-like cells seeded on titanium or zirconia surfaces. Cells were laser irradiated with low therapeutical doses at different intervals and the effects of irradiation were evaluated at each time-point. After 3 hours lasered cells showed an enhanced mitogen activity compared to non-lasered control cells and a higher alkaline phosphatase activity, marker of bone formation. At the same time, the mRNA of RUNX2 and OSTERIX, two genes involved in osteoblast differentiation, showed a clear decrease in lasered cells. This reached the lowest value 6 to 12 hours after irradiation, after which the transcripts started to increase, indicating that the laser treatment did promote the osteogenic potential of growth-induced cells. These results indicate that Low Level Laser Treatment (LLLT) stimulates osteogenic cell proliferation.
F R Grassi; F Ciccolella; G D'Apolito; F Papa; A Iuso; A E Salzo; R Trentadue; G M Nardi; M Scivetti; M De Matteo; F Silvestris; A Ballini; F Inchingolo; G Dipalma; M Dipalma; S Scacco; S Tetè
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biological regulators and homeostatic agents     Volume:  25     ISSN:  0393-974X     ISO Abbreviation:  J. Biol. Regul. Homeost. Agents     Publication Date:    2011 Oct-Dec
Date Detail:
Created Date:  2012-01-05     Completed Date:  2012-02-09     Revised Date:  2012-05-14    
Medline Journal Info:
Nlm Unique ID:  8809253     Medline TA:  J Biol Regul Homeost Agents     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  603-14     Citation Subset:  IM    
Department of Clinical Methodology and Medical Surgery Technology, University of Bari, Bari, Italy.
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MeSH Terms
Bone Matrix / radiation effects
Cell Proliferation / radiation effects
Cell Respiration / radiation effects
Cells, Cultured
Core Binding Factor Alpha 1 Subunit / genetics
Laser Therapy, Low-Level*
Middle Aged
Osteoblasts / radiation effects*
Osteogenesis / radiation effects*
Transcription Factors / genetics
Reg. No./Substance:
0/Core Binding Factor Alpha 1 Subunit; 0/RUNX2 protein, human; 0/Sp7 protein, human; 0/Transcription Factors
Erratum In:
J Biol Regul Homeost Agents. 2012 Jan-Mar;26(1):3 p following 163
Note: Dipalma, M [corrected to Dipalma, G]

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