| Effect of loss of T-DNA genes on MIA biosynthetic pathway gene regulation and alkaloid accumulation in Catharanthus roseus hairy roots. | |
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MedLine Citation:
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PMID: 20625736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hairy roots are generated by integration of T-DNA in host plant genome from root inducing (Ri) plasmid of Agrobacterium rhizogenes and have been utilized for production of secondary metabolites in different plant systems. In Catharanthus roseus, hairy roots are known to show different morphologies, growth patterns, and alkaloid contents. It is also known that during transformation, there is a differential loss of a few T-DNA genes. To decipher the effect of loss of T-DNA genes on the various aspects of hairy roots, ten hairy root clones were analyzed for the presence or absence of T-DNA genes and its implications. It was found that the loss of a few ORFs drastically affects the growth and morphological patterns of hairy roots. The absence of T(R)-DNA from hairy roots revealed increased transcript accumulation and higher alkaloid concentrations, whereas callusing among hairy root lines led to decreased transcript and alkaloid accumulation. Significantly higher expression of MIA biosynthetic pathway genes and low abundance of regulator transcripts in hairy root clones in comparison with non-transformed control roots were also observed. This study indicates that it is not only the integration of T-DNA at certain region of host plant genome but also the presence or absence of important ORFs that affects the expression patterns of MIA biosynthetic pathway genes, regulators, and accumulation of specific alkaloids. |
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Authors:
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Jyoti Taneja; Monika Jaggi; Dhammaprakash Pandhari Wankhede; Alok Krishna Sinha |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-13 |
Journal Detail:
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Title: Plant cell reports Volume: 29 ISSN: 1432-203X ISO Abbreviation: Plant Cell Rep. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-14 Completed Date: 2010-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 1119-29 Citation Subset: IM |
Affiliation:
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National Institute of Plant Genome Research, PO Box 10531, Aruna Asaf Ali Marg, New Delhi 110 067, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Catharanthus
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genetics*,
metabolism DNA, Bacterial / genetics* DNA, Plant / genetics Gene Expression Regulation, Plant Genes, Plant Open Reading Frames Plant Roots / genetics, growth & development, metabolism* Secologanin Tryptamine Alkaloids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Plant; 0/Secologanin Tryptamine Alkaloids; 0/T-DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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