Document Detail

Effect of local tumor removal and retained oncolysate on lung metastasis.
MedLine Citation:
PMID:  1405587     Owner:  NLM     Status:  MEDLINE    
We reported earlier that oncolysate retained in the excision wound of a local tumor inhibits growth of remote tumor in the rat. We further studied this effect on pulmonary metastasis. C57BL/6 mice were given B16 melanoma F10 cells subcutaneously into the gluteal area (Day 0) and then intravenously on Day 10. On Day 14, mice were divided into four groups. Group 1 received a sham operation and no further treatment. Tumors were excised in the remaining mice. Group 2 received tumor excision alone. Groups 3 and 4 received injections of freeze-lysed tumor cells (TC) and lysate modified (PTC) with a hapten, L-phenylalanine mustard (PhM), respectively, into excision wounds. On Day 24, metastases were assessed by determining metastatic burden. Average diameters of excised tumors in repeated experiments ranged from 8.7 to 10.9 mm. In repeat experiments, pulmonary metastatic burden increased by as much as 52 to 181% in the tumor excised group (Group 2) in comparison with those receiving sham surgery (Group 1). However, metastatic burden was always reduced in Group 3. An even greater reduction was seen in Group 4. To study the possible involvement of macrophages, the production of prostaglandin-E2 (PGE2) and cytotoxicity of macrophages in these animals were examined. It was found that tumor excision enhanced PGE2 production by macrophages and suppressed their cytotoxicity, while TC inoculation prevented both of these changes. An even greater prevention was observed with PTC inoculation. These results indicate an association among macrophage cytotoxicity, PGE2 production of macrophages, and metastasis. In order to clarify the mechanism for these reactions, we did experiments using adherent splenic macrophages from the four groups of animals.(ABSTRACT TRUNCATED AT 250 WORDS)
K Arai; T Asakura; P Nemir
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  53     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-11-02     Completed Date:  1992-11-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  30-8     Citation Subset:  IM    
Department of Surgery, Graduate Hospital, Philadelphia, Pennsylvania 19146.
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MeSH Terms
Cell Survival / drug effects
Dinoprostone / biosynthesis
Lung Neoplasms / pathology,  secondary*
Macrophages / pathology,  physiology*
Melanoma, Experimental / immunology,  secondary*,  surgery*
Melphalan / pharmacology
Mice, Inbred C57BL
Neoplasm Metastasis / pathology*
Reg. No./Substance:
148-82-3/Melphalan; 363-24-6/Dinoprostone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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