| Effect of lipoxygenase inhibition on mucous glycoprotein secretion from chinchilla middle ear epithelial cells in vitro. | |
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MedLine Citation:
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PMID: 8916869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipoxygenase is an enzyme that metabolizes arachidonic acid down to leukotrienes. Recent studies have shown that the enzyme is implicated in mucous glycoprotein (MGP) secretion stimulated by inflammatory mediators in the airways, suggesting its possible role in secretion of MGP from middle ear epithelial cells. To investigate a correlation between MGP secretion and the arachidonic acid metabolites, we examined the effects of nordihydroguaretic acid (NDGA, both a cyclooxygenase and lipoxygenase inhibitor), low-dose indomethacin (an inhibitor of cyclooxygenase), and A63162 (an inhibitor of lipoxygenase) on MGP secretion in cultured chinchilla middle ear epithelial cells. It was found that lipoxygenase inhibition led to reduction of MGP secretion from cultured chinchilla middle ear epithelial cells, while cyclooxygenase inhibition did not. Both cyclooxygenase and lipoxygenase inhibition resulted in profound blockage of MGP secretion in baseline and platelet activating factor-stimulated MGP secretion. It was concluded, therefore, that MGP secretion was linked to arachidonic acid metabolites, especially lipoxygenase products. |
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Authors:
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J Lin; Y Kim; C Less; F Ondrey; S K Juhn |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Annals of otology, rhinology, and laryngology Volume: 105 ISSN: 0003-4894 ISO Abbreviation: Ann. Otol. Rhinol. Laryngol. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1996-12-11 Completed Date: 1996-12-11 Revised Date: 2010-05-07 |
Medline Journal Info:
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Nlm Unique ID: 0407300 Medline TA: Ann Otol Rhinol Laryngol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 916-21 Citation Subset: AIM; IM |
Affiliation:
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Otitis Media Research Center, Department of Otolaryngology, University of Minnesota School of Medicine, Minneapolis, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetamides
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pharmacology* Animals Arachidonic Acid / physiology Cells, Cultured Chinchilla Cyclooxygenase Inhibitors / pharmacology* Ear, Middle / drug effects, secretion* Epithelium / drug effects, secretion Glycoproteins / secretion* Indomethacin / pharmacology* Lipoxygenase Inhibitors / pharmacology* Nordihydroguaiaretic Acid / pharmacology* Platelet Activating Factor / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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P01-DC00133/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acetamides; 0/Cyclooxygenase Inhibitors; 0/Glycoproteins; 0/Lipoxygenase Inhibitors; 0/Platelet Activating Factor; 0/gastric mucus glycoproteins; 111525-11-2/N-hydroxy-N-(1-(4-(phenylmethoxy)phenyl)ethyl)-acetamide; 500-38-9/Nordihydroguaiaretic Acid; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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