Document Detail


Effect of lipoproteins and luteinizing hormone on progesterone production by large and small luteal cells throughout the porcine estrous cycle.
MedLine Citation:
PMID:  3667449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Large and small cells were isolated from porcine corpora lutea (CL) on d 10, 15 or 18 of the estrous cycle. They were incubated 13 to 16 h in cholesterol- and serum-free media and then supplied with 0, 10, 50 or 100 micrograms of either porcine low density lipoprotein (LDL) or porcine high density lipoprotein (HDL). Each dose was supplemented with 0, 10, 50 or 100 ng of porcine LH. Media progesterone (P4) content was assessed immediately before and 2 and 24 h after addition of lipoproteins and LH. Production of P4 by large cells always exceeded that of small cells. Day 10 large cells were stimulated by LDL, unaffected by LH, and either inhibited or unaffected by HDL. No treatment affected d 10 small cells. Day 15 large cells and small cells were stimulated by both lipoproteins (LDL greater than HDL). The large cells were stimulated to a small extent by LH at 2 h (P less than .05). Large cells could be isolated from only two of five preparations of d 18 CL. Day 18 small cells produced a small quantity of P4 in a response which qualitatively resembled that of d 15 small cells. Cell type, cycle stage and lipoprotein (particularly LDL) were the major effectors of P4 production. The minimal response to LH supports the theory of autonomy of the porcine CL with respect to P4 production. Days 10 and 15 bracket the period of commitment to luteolysis, and the nature of P4 production by each cell type changed over that period.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
M M Buhr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal science     Volume:  65     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1987-12-08     Completed Date:  1987-12-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1027-33     Citation Subset:  IM    
Affiliation:
Dept. of Anim. Sci., University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Corpus Luteum / metabolism*
Estrus / metabolism*
Female
Lipoproteins, HDL / pharmacology*
Lipoproteins, LDL / pharmacology*
Luteal Cells / drug effects,  metabolism*
Luteinizing Hormone / pharmacology*
Progesterone / biosynthesis*
Swine / physiology*
Chemical
Reg. No./Substance:
0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 57-83-0/Progesterone; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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