| Effect of lipoic acid on expression of angiogenic factors in diabetic rat retina. | |
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MedLine Citation:
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PMID: 21902786 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: This study evaluated the effect of a lipoic acid on reactive oxygen species (ROS) formation and the simultaneous changes of several angiogenic factors in an experimental diabetic rat retina. Methods: Diabetes was induced chemically by intraperitoneal injection of streptozotocin in 30 Sprague-Dawley rats. After inducing diabetes, lipoic acid (10 mg/kg) was administered to 10 rats orally. The rats were divided into normal, diabetes mellitus (DM), and lipoic acid-treated groups (each group n = 10). The eyeballs were harvested 8 weeks after inducing diabetes. The expression of VEGF, erythropoietin, angiopoietin 1 & 2 and NADPH oxidase was examined in the rat retina using RT-PCR, and western blot. Superoxide formation was examined using dihidroethidium (DHE) stain. Results: DHE analyses showed increased superoxide formation in the retina of the diabetic group. The superoxide formation was suppressed with lipoic acid treatment. Western blot analysis showed that NADPH oxidase was decreased in the diabetic group and returned to normal level in the lipoic acid treated group. Treatment with lipoic acid blocked hyperglycemia induced increases of VEGF, angiopoietin 2 and erythropoietin shown by RT-PCR and western blot analysis. Conclusions: Lipoic acid treatment suppressed expression of VEGF, angiopoietin 2 and erythropoietin via blockade of superoxide formation. Antioxidant treatment is suspected to have an antiangiogenic effect. |
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Authors:
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Sul Gee Lee; Chang Gun Lee; Il Han Yun; Dae Young Hur; Jae Wook Yang; Hyun Woong Kim |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-8 |
Journal Detail:
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Title: Clinical & experimental ophthalmology Volume: - ISSN: 1442-9071 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100896531 Medline TA: Clin Experiment Ophthalmol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 The Authors. Journal compilation © 2011 Royal Australian and New Zealand College of Ophthalmologists. |
Affiliation:
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Department of Ophthalmology, College of Medicine, Inje University, Busan Paik Hospital, Busan, Republic of Korea Kangnam Hanul Ophthalmic Clinic, Seoul, Republic of Korea Department of Anatomy and Cell Biology, Inje University, Busan Paik Hospital, Busan, Republic of Korea. |
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