| Effect of lipid composition on rat liver nuclear membrane fluidity. | |
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MedLine Citation:
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PMID: 9377796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nuclear membrane fluidity is measured in rat liver by use of the fluorescence anisotropy of two probes: diphenylhexatriene and its cationic derivative trimethylammonium-diphenylhexatriene. It has been shown that, in 2-month-old rat liver cells, the bilayer surface is less fluid than the hydrophobic core. The fluidity was higher in 6-day-old rat liver nuclei, in which both the amount of cholesterol and the cholesterol/phospholipid ratio decreased. The influence of the single phospholipids, and in particular of phosphatidylcholine, has been studied by increasing the phosphatidylcholine with a choline base exchange reaction in isolated nuclear membranes. After this reaction, the fluorescence anisotropy of the bilayer surface increased, whereas at the hydrophobic core it decreased. Analysis of fatty acid composition shows an increase of phosphatidylcholine unsaturated fatty acids. The results show that the fluidity of nuclear membranes changes in relation to the lipid content and to the fatty acid composition. The role of nuclear membrane fluidity in cell function is discussed. |
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Authors:
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E Albi; M L Tomassoni; M Viola-Magni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biochemistry and function Volume: 15 ISSN: 0263-6484 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1997-11-10 Completed Date: 1997-11-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 181-90 Citation Subset: IM |
Affiliation:
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Institute of General Pathology, University of Perugia, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Animals, Newborn Cholesterol / analysis, metabolism Choline / metabolism Diphenylhexatriene / analogs & derivatives Fatty Acids, Unsaturated / analysis, metabolism Female Fluorescence Polarization Fluorescent Dyes Liver / metabolism* Male Membrane Fluidity / physiology* Nuclear Envelope / chemistry, metabolism* Phospholipids / analysis, metabolism* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Fluorescent Dyes; 0/Phospholipids; 1720-32-7/Diphenylhexatriene; 57-88-5/Cholesterol; 62-49-7/Choline; 71316-28-4/1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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