Document Detail

Effect of lipid composition on rat liver nuclear membrane fluidity.
MedLine Citation:
PMID:  9377796     Owner:  NLM     Status:  MEDLINE    
Nuclear membrane fluidity is measured in rat liver by use of the fluorescence anisotropy of two probes: diphenylhexatriene and its cationic derivative trimethylammonium-diphenylhexatriene. It has been shown that, in 2-month-old rat liver cells, the bilayer surface is less fluid than the hydrophobic core. The fluidity was higher in 6-day-old rat liver nuclei, in which both the amount of cholesterol and the cholesterol/phospholipid ratio decreased. The influence of the single phospholipids, and in particular of phosphatidylcholine, has been studied by increasing the phosphatidylcholine with a choline base exchange reaction in isolated nuclear membranes. After this reaction, the fluorescence anisotropy of the bilayer surface increased, whereas at the hydrophobic core it decreased. Analysis of fatty acid composition shows an increase of phosphatidylcholine unsaturated fatty acids. The results show that the fluidity of nuclear membranes changes in relation to the lipid content and to the fatty acid composition. The role of nuclear membrane fluidity in cell function is discussed.
E Albi; M L Tomassoni; M Viola-Magni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  15     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-11-10     Completed Date:  1997-11-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  181-90     Citation Subset:  IM    
Institute of General Pathology, University of Perugia, Italy.
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MeSH Terms
Age Factors
Animals, Newborn
Cholesterol / analysis,  metabolism
Choline / metabolism
Diphenylhexatriene / analogs & derivatives
Fatty Acids, Unsaturated / analysis,  metabolism
Fluorescence Polarization
Fluorescent Dyes
Liver / metabolism*
Membrane Fluidity / physiology*
Nuclear Envelope / chemistry,  metabolism*
Phospholipids / analysis,  metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Fluorescent Dyes; 0/Phospholipids; 1720-32-7/Diphenylhexatriene; 57-88-5/Cholesterol; 62-49-7/Choline; 71316-28-4/1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene

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