| Effect of a leukotriene inhibitor (MK886) on nitric oxide and hydrogen peroxide production by macrophages of acutely and chronically stressed mice. | |
| | |
MedLine Citation:
|
PMID: 17883896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We evaluated the effect of a leukotriene inhibitor (MK886) on nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) production by peritoneal macrophages of mice subjected to acute and chronic stress. Acute stress was induced by keeping mice immobilized in a tube for 2 h. Chronic stress was induced over a 7-day period by the same method, but with increasing duration of immobilization. The effects of MK886 were investigated in-vitro after incubation with peritoneal macrophages, and in-vivo by submitting mice to stress and treating them daily with MK886. Supernatants of macrophage cultures were collected for NO determination and adherent cells were used for H(2)O(2) determination. Macrophages from mice submitted to acute or chronic stress showed no alterations in H(2)O(2) production. However, macrophages of acutely and chronically stressed mice showed inhibition of NO after incubation with MK886 in-vitro. Administration of MK886 to chronically stressed mice increased generation of H(2)O(2) and inhibited production of NO. Our data suggest an important role of leukotrienes in NO synthesis, which is important in controlling replication of several infectious agents, mainly in stressed and immunosuppressed animals. |
| | |
Authors:
|
José Maurício Sforcin; Gladston A Nunes; Fabiane Missima; Anderson Sá-Nunes; Lúcia H Faccioli |
Related Documents
:
|
1849216 - Stress-induced neuroinvasiveness of a neurovirulent noninvasive sindbis virus in cold o... 15184356 - Stress-induced pressor and corticosterone responses in oxytocin-deficient mice. 21436586 - Mouse and human neutrophils induce anaphylaxis. 11682116 - Repeated exposure to rats has persistent genotype-dependent effects on learning and loc... 8390816 - Conditions of formation, purification, and characterization of an alpha-galactosidase o... 3080566 - Initiation and promotion of experimental oral mucosal carcinogenesis in mice. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of pharmacy and pharmacology Volume: 59 ISSN: 0022-3573 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2007 Sep |
Date Detail:
|
Created Date: 2007-09-21 Completed Date: 2008-01-08 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
|
Languages: eng Pagination: 1249-54 Citation Subset: IM |
Affiliation:
|
Departamento de Microbiologia e Imunologia, Instituto de Biociências, UNESP, Botucatu, SP 18618-000, Brazil. sforcin@ibb.unesp.br |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Glucose / metabolism Corticosterone / blood, metabolism Hydrogen Peroxide / metabolism Indoles / administration & dosage, pharmacology* Leukotriene Antagonists / administration & dosage, pharmacology* Lipoxygenase Inhibitors / administration & dosage, pharmacology* Macrophages, Peritoneal / drug effects*, metabolism Male Mice Mice, Inbred BALB C Nitric Oxide / biosynthesis Stress, Physiological / physiopathology Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Blood Glucose; 0/Indoles; 0/Leukotriene Antagonists; 0/Lipoxygenase Inhibitors; 10102-43-9/Nitric Oxide; 118414-82-7/L 663536; 50-22-6/Corticosterone; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of tetracycline on pancreas and liver function of adult male albino rats.
Next Document: Inhibitory mechanisms of gabapentin, an antiseizure drug, on platelet aggregation.