Document Detail

Effect of leptin on polymorphonuclear leucocyte functions in healthy subjects and haemodialysis patients.
MedLine Citation:
PMID:  21216885     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Dysfunction of polymorphonuclear leucocytes (PMNLs) in end-stage renal disease (ESRD) patients contributes to a diminished immune defence. The serum levels of leptin are elevated in patients with ESRD. We analysed in vitro effects of leptin on PMNLs from healthy subjects (HS; n = 12) and haemodialysis (HD) patients (n = 15) before and after HD.
METHODS: PMNL oxidative burst and phagocytosis were tested by flow cytometry in whole blood. Chemotaxis of isolated PMNLs was assessed by the under-agarose method. To assess the involvement of leptin in PMNL signalling pathways, signal transduction inhibitors were used and the activity of intracellular kinases was investigated by western blotting, in vitro kinase assays and the Luminex technology.
RESULTS: Increasing the leptin level in the blood of HS leads to a reduced activation of the oxidative burst by Escherichia coli and phorbol 12-myristate 13-acetate. Activation of the oxidative burst is reduced in the blood of HD patients and the addition of leptin does not lead to further PMNL inhibition. Leptin at a concentration measured in HD patients significantly reduces the chemotaxis of PMNLs from HS but had no effect on PMNLs from ESRD patients before and also after HD treatment with high-flux dialysers. The phosphoinositide 3-kinase/Akt pathway is involved in the inhibitory effects of leptin.
CONCLUSIONS: In the presence of leptin, PMNLs from HS and HD patients respond differently to stimuli. The lack of response to leptin in PMNLs from HD patients cannot be influenced by HD.
Gerald Cohen; Jana Raupachova; Dalibor Ilic; Johannes Werzowa; Walter H Hörl
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-01-07
Journal Detail:
Title:  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association     Volume:  26     ISSN:  1460-2385     ISO Abbreviation:  Nephrol. Dial. Transplant.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-28     Completed Date:  2011-10-31     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  8706402     Medline TA:  Nephrol Dial Transplant     Country:  England    
Other Details:
Languages:  eng     Pagination:  2271-81     Citation Subset:  IM    
Department of Internal Medicine, Division of Nephrology and Hypertension, University of Cincinnati, Cincinnati, OH, USA.
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MeSH Terms
Blotting, Western
Case-Control Studies
Glucose / metabolism
I-kappa B Kinase / metabolism
Kidney Failure, Chronic / metabolism*,  pathology,  therapy*
Leptin / blood*
Neutrophils / physiology*
Phagocytosis / drug effects*
Renal Dialysis*
Respiratory Burst / drug effects*
Risk Factors
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
1K23DK083528-01/DK/NIDDK NIH HHS; 1R43DK077552-01A/DK/NIDDK NIH HHS; 1U01DK082218-0/DK/NIDDK NIH HHS; 2R01EB004527-05/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Leptin; 16561-29-8/Tetradecanoylphorbol Acetate; 50-99-7/Glucose; EC B Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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