| Effect of lactoferrin in combination with penicillin on the morphology and the physiology of Staphylococcus aureus isolated from bovine mastitis. | |
| | |
MedLine Citation:
|
PMID: 12086049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The objective of the present study was to evaluate the therapeutic potential of bovine lactoferrin or lactoferricin in combination with penicillin G against Staphylococcus aureus. Minimal inhibitory concentrations of lactoferrin, lactoferricin, penicillin, and combinations of lactoferrin or lactoferricin with penicillin were determined for 15 S. aureus strains including several strains resistant to beta-lactam antibiotics. The fractional inhibitory concentration index indicated a synergistic effect between lactoferrin and penicillin. Combination of lactoferrin with penicillin increased the inhibitory activity of penicillin by two- to fourfold and reduced the growth rate in S. aureus strains tested, whereas the increase in the inhibitory activity of lactoferrin by penicillin was 16- to 64-fold. The addition of iron to the medium containing a combination of penicillin and lactoferrin had no effect on growth inhibition. Electron microscopy revealed that concentration below the minimal inhibitory concentrations of penicillin induced important ultrastructure alterations, which were further enhanced by the presence of lactoferrin. When S. aureus cells were grown in the presence of a combination of penicillin and lactoferrin, changes in the protein profile of the bacteria, including the disappearance of several protein bands due to the presence of lactoferrin, were observed. These data suggest that bovine lactoferrin or lactoferricin in combination with beta-lactam antibiotics can increase the antibacterial activity of these antibiotics against S. aureus resistant to antibiotics. |
| | |
Authors:
|
M S Diarra; D Petitclerc; P Lacasse |
Related Documents
:
|
3146599 - Enzyme immunoassay (eia) in the rapid diagnosis of gonorrhoea. 15150169 - Molecular characterization of penicillin non-susceptible streptococcus pneumoniae in ch... 6271689 - Serotypes and antimicrobial susceptibility of streptococcus pneumoniae in west germany. 11733469 - Evolution in the trends of antimicrobial resistance in neisseria gonorrhoeae isolated i... 12081169 - Gonorrhoea in london: usefulness of first line therapies. 10488179 - The three major spanish clones of penicillin-resistant streptococcus pneumoniae are the... 20526539 - Antimicrobial activity of topical skin pharmaceuticals - an in vitro study. 2851499 - Synthesis of the major inner capsid protein vp6 of the human rotavirus wa in escherichi... 21908289 - Minimum inhibitory concentrations of selected antimicrobial agents for moraxella bovocu... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of dairy science Volume: 85 ISSN: 0022-0302 ISO Abbreviation: J. Dairy Sci. Publication Date: 2002 May |
Date Detail:
|
Created Date: 2002-06-27 Completed Date: 2002-12-20 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 1141-9 Citation Subset: IM |
Affiliation:
|
Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Lennoxville, QC. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacterial Proteins / analysis Cattle Drug Resistance, Microbial Drug Synergism Female Lactoferrin / administration & dosage* Mastitis, Bovine / microbiology* Microscopy, Electron Penicillin G / administration & dosage* Penicillins / administration & dosage* Staphylococcus aureus / cytology, drug effects*, ultrastructure |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Lactoferrin; 0/Penicillins; 61-33-6/Penicillin G |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of Staphylococcus aureus strain-type on mammary quarter milk somatic cell count and N-acet...
Next Document: The relationship between estrous behavioral score and time of ovulation in dairy cattle.