Document Detail


Effect of the kinetin-naphthaleneacetic acid interaction upon total RNA and protein in senescing detached leaves.
MedLine Citation:
PMID:  5700020     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein. Kinetin increased the net uptake of (14)C-labeled orotic acid and leucine. Naphthaleneacetic acid decreased the effect of kinetin on net uptake after long treatment, but in short-time treatments the auxin increased the effect of kinetin on net uptake. Results of long (24 hr) treatments indicated a general synchrony between the loss of RNA, protein, and chlorophyll. Naphthaleneacetic acid reduced the stabilizing effect of kinetin upon chlorophyll content and upon the content and synthesis of RNA. In short-time experiments, however, RNA content and synthesis were transiently increased by kinetin, and further increased by kinetin plus naphthaleneacetic acid, while chlorophyll content decreased in the presence of kinetin and decreased further in the presence of kinetin plus naphthaleneacetic acid. Actinomycin-D accelerated the loss of chlorophyll, RNA and protein and strongly depressed the rate of RNA synthesis. In the presence of actinomycin-D the stabilizing effect of kinetin upon RNA was substantially reduced. In contrast, the chlorophyll and protein contents remained higher than in the control. Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA, nor did it negate the effect of kinetin upon incorporation. The failure of synchrony between changes in chlorophyll and RNA does not substantiate the proposal that kinetin regulates senescence by a direct effect upon DNA-dependent RNA synthesis.
Authors:
G J VonAbrams; H K Pratt
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  43     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1968 Aug 
Date Detail:
Created Date:  1969-01-10     Completed Date:  1969-01-10     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1271-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Naphthaleneacetic Acids / pharmacology*
Plant Growth Regulators / pharmacology*
Plant Proteins / metabolism*
Plants / metabolism*
RNA / metabolism*
Chemical
Reg. No./Substance:
0/Naphthaleneacetic Acids; 0/Plant Growth Regulators; 0/Plant Proteins; 63231-63-0/RNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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