Document Detail

Effect of ischemic preconditioning on the expression of putative neuroprotective genes in the rat brain.
MedLine Citation:
PMID:  12106696     Owner:  NLM     Status:  MEDLINE    
Previous studies have demonstrated that sublethal ischemic insults protect from subsequent ischemia in the intact brain. There are two windows for the induction of tolerance by ischemic preconditioning (IPC). One occurs within 1 h following IPC, and the other one develops from 1 to 3 days after IPC. The goal of this study was to determine whether IPC neuroprotection may be mediated by expression of known neuroprotective genes and to characterize the temporal and spatial expression patterns of these genes. IPC was produced by bilateral carotid artery occlusions and hypotension (50 mmHg) for 2 min. After various survival times, the expression of MAP-2, brain-derived neurotrophic factor (BDNF), c-jun, c-fos, nerve growth factor (NGF) and HSP70 was assessed by in situ hybridization of coronal brain sections with 35S labeled probes. BDNF, NGF, and c-jun were significantly upregulated in the hippocampus. c-fos was detected in the hippocampus, cortex and striatum. HSP70 mRNA was induced in the cortex, hippocampus, striatum, and thalamus. MAP-2 showed no change in expression, confirming previous studies that no cell death occurs following IPC. The increase in expression of these stress-related, neurotrophic and immediate early genes in response to a mild preconditioning insult may help mediate the protection of vulnerable neurons to subsequent lethal ischemic insults.
Jessie Truettner; Raul Busto; Weizhao Zhao; Myron D Ginsberg; Miguel A Pérez-Pinzón
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  103     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-07-10     Completed Date:  2002-11-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  106-15     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science B.V.
The Cerebral Vascular Disease Research Center, Department of Neurology (D4-5), University of Miami School of Medicine, P.O. Box 16960, Miami, FL 33101, USA.
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MeSH Terms
Brain Chemistry / genetics*
Brain Ischemia / physiopathology*
Brain-Derived Neurotrophic Factor / genetics
Gene Expression
Genes, Immediate-Early / physiology
HSP70 Heat-Shock Proteins / genetics
In Situ Hybridization
Ischemic Preconditioning*
Microtubule-Associated Proteins / genetics
Nerve Growth Factor / genetics
Proto-Oncogene Proteins c-fos / genetics
Proto-Oncogene Proteins c-jun / genetics
RNA, Messenger / analysis
Rats, Wistar
Grant Support
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/HSP70 Heat-Shock Proteins; 0/Microtubule-Associated Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 9061-61-4/Nerve Growth Factor

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