Document Detail


Effect of ischemia and reperfusion on sarcoplasmic reticulum calcium uptake.
MedLine Citation:
PMID:  1394874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the mechanism underlying postischemic cardiac dysfunction (myocardial stunning), contractility and adenine nucleotide metabolism were studied in three groups of isolated perfused rabbit hearts (control, ischemic, and reperfused), whereas Ca2+ uptake by the sarcoplasmic reticulum (SR) was measured in homogenates obtained from them. The hearts were Langendorff-perfused under constant pressure with Krebs-Henseleit solution at 37 degrees C. Global normothermic ischemia was produced by closing the perfusion line. In the reperfused group, after 15 minutes of ischemia, Krebs-Henseleit solution was perfused for 10 minutes. Developed left ventricular pressure (control, 104 +/- 6.3 mm Hg) and left ventricular dP/dt (2,063 +/- 256.6 mm Hg.sec-1) were significantly decreased in reperfused hearts (left ventricular pressure, 78 +/- 5.9 mm Hg; left ventricular dP/dt, 1,339 +/- 216.3 mm Hg.sec-1). Myocardial ATP content (control, 13.6 +/- 0.98 mumol/g dry wt) decreased during ischemia (4.5 +/- 1.23 mumol/g) but was restored to control level on reperfusion (11.8 +/- 0.68 mumol/g). Maximum velocity of Ca2+ uptake by the SR (Vmax) (control, 49.3 +/- 2.54 nmol.min-1 x mg-1) was significantly depressed by ischemia (36.3 +/- 1.94 nmol.min-1 x mg-1) but was restored to the control value after a 10-minute reperfusion (45.3 +/- 0.79 nmol.min-1 x mg-1). Apparent dissociation constant KCa and the Hill coefficient for Ca2+ uptake were not different between control, ischemia, and reperfusion. To test for the possible role of the SR Ca(2+)-release channel in the effect of ischemia and reperfusion, we measured Ca2+ uptake after incubation of homogenates with 610 microM ryanodine.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
P Kaplan; M Hendrikx; M Mattheussen; K Mubagwa; W Flameng
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Circulation research     Volume:  71     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1992-11-16     Completed Date:  1992-11-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1123-30     Citation Subset:  IM    
Affiliation:
Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides / metabolism
Animals
Calcium / metabolism*
Myocardial Contraction
Myocardial Ischemia / metabolism*
Myocardial Reperfusion*
Myocardium / metabolism
Rabbits
Sarcoplasmic Reticulum / metabolism*
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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