| Effect of ischemia and reperfusion on sarcoplasmic reticulum calcium uptake. | |
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MedLine Citation:
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PMID: 1394874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the mechanism underlying postischemic cardiac dysfunction (myocardial stunning), contractility and adenine nucleotide metabolism were studied in three groups of isolated perfused rabbit hearts (control, ischemic, and reperfused), whereas Ca2+ uptake by the sarcoplasmic reticulum (SR) was measured in homogenates obtained from them. The hearts were Langendorff-perfused under constant pressure with Krebs-Henseleit solution at 37 degrees C. Global normothermic ischemia was produced by closing the perfusion line. In the reperfused group, after 15 minutes of ischemia, Krebs-Henseleit solution was perfused for 10 minutes. Developed left ventricular pressure (control, 104 +/- 6.3 mm Hg) and left ventricular dP/dt (2,063 +/- 256.6 mm Hg.sec-1) were significantly decreased in reperfused hearts (left ventricular pressure, 78 +/- 5.9 mm Hg; left ventricular dP/dt, 1,339 +/- 216.3 mm Hg.sec-1). Myocardial ATP content (control, 13.6 +/- 0.98 mumol/g dry wt) decreased during ischemia (4.5 +/- 1.23 mumol/g) but was restored to control level on reperfusion (11.8 +/- 0.68 mumol/g). Maximum velocity of Ca2+ uptake by the SR (Vmax) (control, 49.3 +/- 2.54 nmol.min-1 x mg-1) was significantly depressed by ischemia (36.3 +/- 1.94 nmol.min-1 x mg-1) but was restored to the control value after a 10-minute reperfusion (45.3 +/- 0.79 nmol.min-1 x mg-1). Apparent dissociation constant KCa and the Hill coefficient for Ca2+ uptake were not different between control, ischemia, and reperfusion. To test for the possible role of the SR Ca(2+)-release channel in the effect of ischemia and reperfusion, we measured Ca2+ uptake after incubation of homogenates with 610 microM ryanodine.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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P Kaplan; M Hendrikx; M Mattheussen; K Mubagwa; W Flameng |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Circulation research Volume: 71 ISSN: 0009-7330 ISO Abbreviation: Circ. Res. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1992-11-16 Completed Date: 1992-11-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1123-30 Citation Subset: IM |
Affiliation:
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Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Nucleotides
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metabolism Animals Calcium / metabolism* Myocardial Contraction Myocardial Ischemia / metabolism* Myocardial Reperfusion* Myocardium / metabolism Rabbits Sarcoplasmic Reticulum / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Adenine Nucleotides; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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