| Effect of iron on polymorphonuclear granulocyte phagocytic capacity: role of oxidation state and effect of ascorbic acid. | |
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MedLine Citation:
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PMID: 3219299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been shown that iron (III) impairs the function of polymorphonuclear granulocytes (PMN). We have studied the effect of iron (II), on the membrane function of PMN, by assessing the uptake of radiolabelled Staphylococcus aureus by these cells. Iron (II), significantly impaired PMN phagocytic function. Addition of ascorbic acid reduced uptake further. Ferrous ascorbate, molar ratio 1:20, impaired phagocytic capacity of PMN significantly at iron concentrations as low as 1-10 microM. The toxic effect of iron (II) was not observed when desferrioxamine or transferrin was present in the incubation medium. The oxygen-free radical scavengers thiourea, mannitol and catalase prevented toxicity mediated by ferrous ammoniumsulphate but not by ferrous ascorbate (molar ratio of 1:20). Although high concentrations of ascorbic acid inhibited the generation of .OH and also the formation of the DMPO-.OH adduct by zymosan stimulated PMN, toxicity of iron increased. Iron (II) impaired the uptake of S. aureus by PMN of a patient with chronic granulomatous disease while iron (III) did not. Iron mediated impairment of PMN function is not only a result of the generation of toxic oxygen metabolites but also of direct interaction of iron (II) or an iron (II)-oxygen intermediate with molecules of the cell membrane. |
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Authors:
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I M Hoepelman; E Y Jaarsma; J Verhoef; J J Marx |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: British journal of haematology Volume: 70 ISSN: 0007-1048 ISO Abbreviation: Br. J. Haematol. Publication Date: 1988 Dec |
Date Detail:
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Created Date: 1989-03-21 Completed Date: 1989-03-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372544 Medline TA: Br J Haematol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 495-500 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University Hospital Utrecht, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Ascorbic Acid / pharmacology* Ferrous Compounds / pharmacology* Granulomatous Disease, Chronic / blood Humans Neutrophils / drug effects* Oxidation-Reduction Phagocytosis / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Ferrous Compounds; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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