Document Detail

Effect of iron on polymorphonuclear granulocyte phagocytic capacity: role of oxidation state and effect of ascorbic acid.
MedLine Citation:
PMID:  3219299     Owner:  NLM     Status:  MEDLINE    
It has been shown that iron (III) impairs the function of polymorphonuclear granulocytes (PMN). We have studied the effect of iron (II), on the membrane function of PMN, by assessing the uptake of radiolabelled Staphylococcus aureus by these cells. Iron (II), significantly impaired PMN phagocytic function. Addition of ascorbic acid reduced uptake further. Ferrous ascorbate, molar ratio 1:20, impaired phagocytic capacity of PMN significantly at iron concentrations as low as 1-10 microM. The toxic effect of iron (II) was not observed when desferrioxamine or transferrin was present in the incubation medium. The oxygen-free radical scavengers thiourea, mannitol and catalase prevented toxicity mediated by ferrous ammoniumsulphate but not by ferrous ascorbate (molar ratio of 1:20). Although high concentrations of ascorbic acid inhibited the generation of .OH and also the formation of the DMPO-.OH adduct by zymosan stimulated PMN, toxicity of iron increased. Iron (II) impaired the uptake of S. aureus by PMN of a patient with chronic granulomatous disease while iron (III) did not. Iron mediated impairment of PMN function is not only a result of the generation of toxic oxygen metabolites but also of direct interaction of iron (II) or an iron (II)-oxygen intermediate with molecules of the cell membrane.
I M Hoepelman; E Y Jaarsma; J Verhoef; J J Marx
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of haematology     Volume:  70     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-03-21     Completed Date:  1989-03-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  495-500     Citation Subset:  IM    
Department of Internal Medicine, University Hospital Utrecht, The Netherlands.
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MeSH Terms
Ascorbic Acid / pharmacology*
Ferrous Compounds / pharmacology*
Granulomatous Disease, Chronic / blood
Neutrophils / drug effects*
Phagocytosis / drug effects*
Reg. No./Substance:
0/Ferrous Compounds; 50-81-7/Ascorbic Acid

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