| Effect of iron chelators on the cytotoxic and genotoxic action of hyperoxia in Chinese hamster ovary cells. | |
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MedLine Citation:
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PMID: 1372685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The iron chelators o-phenanthroline and desferrioxamine were tested for their ability to protect Chinese hamster ovary cells against the cytotoxic and genotoxic effects of normobaric hyperoxia. Desferrioxamine added at sub-toxic concentrations (up to 2.5 microM) over a period of several days had no protective effect on hyperoxia-induced clonogenic cell killing and growth inhibition. The clastogenic effect of hyperoxia was strongly potentiated by desferrioxamine, while the induction of sister-chromatid exchanges (SCEs) by hyperoxia was unaffected. Similarly, o-phenanthroline (up to 0.25 microM) had no protective effect on hyperoxia-induced cell killing, growth inhibition, and SCE induction, while also this compound potentiated the clastogenic effect of hyperoxia. These results do not support a critical role for cellular iron in the mechanism of toxicity by normobaric hyperoxia in CHO cells. However, the results may still be consistent with a critical involvement of particular iron fraction(s) not susceptible to the chelators used. Furthermore, our results show that concentrations of iron chelators known to protect against short-term (up to 1 h) toxic exposure to oxidative stress become toxic themselves when applied chronically, i.e., in the order of days. |
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Authors:
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J J Gille; C G van Berkel; H Joenje |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mutation research Volume: 275 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1992 Jan |
Date Detail:
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Created Date: 1992-04-30 Completed Date: 1992-04-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 31-9 Citation Subset: IM |
Affiliation:
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Department of Human Genetics, Free University, Amsterdam, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cell Survival Chromosome Aberrations Cricetinae Deferoxamine / pharmacology Hydrogen Peroxide / pharmacology Iron Chelating Agents / pharmacology* Oxygen / antagonists & inhibitors* Phenanthrolines / pharmacology Sister Chromatid Exchange |
| Chemical | |
Reg. No./Substance:
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0/Iron Chelating Agents; 0/Phenanthrolines; 66-71-7/1,10-phenanthroline; 70-51-9/Deferoxamine; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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