Document Detail

Effect of ipriflavone on expression of markers characteristic of the osteoblast phenotype in rat bone marrow stromal cell culture.
MedLine Citation:
PMID:  8191934     Owner:  NLM     Status:  MEDLINE    
The effects of ipriflavone on cellular proliferation and differentiation of osteoblasts were investigated using stromal cells isolated from the femoral bone marrow of young rats. To induce the formation of mineralized bone-like tissue in vitro, the cells were cultured in the presence of beta-glycerophosphate and dexamethasone. Ipriflavone was added when subculturing was started. After 14 days of culturing with ipriflavone (10(-7)-10(-5) M), increases in both the alkaline phosphatase activity and the hydroxyproline content per culture dish and a slight decrease in the saturated cell density were observed. Furthermore, continuous treatment with ipriflavone for 14-33 days resulted in an increase in the area of bone-like mineralized tissue accompanied by an increase in the secretion of osteocalcin. When culture medium lacking dexamethasone was used, rat bone marrow stromal cells neither differentiated into osteoblasts nor formed bone-like tissue, and under these conditions, ipriflavone had no effect on the proliferation or the phenotypic expression of the cells. These results suggest that ipriflavone directly stimulates markers of the osteoblast phenotype at a certain stage in bone formation without affecting undifferentiated cells that have not been committed to the osteogenic lineage.
K Notoya; K Yoshida; R Tsukuda; S Taketomi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research     Volume:  9     ISSN:  0884-0431     ISO Abbreviation:  J. Bone Miner. Res.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-06-23     Completed Date:  1994-06-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8610640     Medline TA:  J Bone Miner Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  395-400     Citation Subset:  IM; S    
Department III, Pharmaceutical Research Laboratories II, Takeda Chemical Industries, Ltd., Osaka, Japan.
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MeSH Terms
Alkaline Phosphatase / metabolism
Bone Development / drug effects
Bone Marrow / drug effects*
Bone Marrow Cells
Calcification, Physiologic / drug effects
Cell Differentiation / drug effects
Cell Division / drug effects
Cells, Cultured
Dexamethasone / metabolism,  pharmacology
Glycerophosphates / metabolism,  pharmacology
Hydroxyproline / metabolism
Isoflavones / pharmacology*
Osteoblasts / cytology,  drug effects*,  metabolism
Osteocalcin / secretion
Stromal Cells / cytology,  drug effects
Reg. No./Substance:
0/Glycerophosphates; 0/Isoflavones; 104982-03-8/Osteocalcin; 17181-54-3/beta-glycerophosphoric acid; 35212-22-7/ipriflavone; 50-02-2/Dexamethasone; 51-35-4/Hydroxyproline; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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