Document Detail


Effect of ionizing radiation on chemical and biological properties of Salmonella minnesota R595 lipopolysaccharide.
MedLine Citation:
PMID:  7185268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipopolysaccharide of the Salmonella minnesota Re mutant R595 was irradiated with 60Co gamma doses of 50, 100, 150 and 200 kGy. The irradiated preparations were less toxic, less active in the Shwartzman reaction and as activators of the complement system, but they had retained the protection activity against the lethal action of endotoxin. The irradiation resulted in a dose-dependent decrease in the amounts of constituents (glucosamine, KDO, fatty acids) of the original lipopolysaccharide. With increasing irradiation doses increasing amounts of the irradiated material became dialysable (up to 21% in the 200 kGy sample). Only 50% of total fatty acids were present in the 200 kGy preparation compared to the parent lipopolysaccharide. The degradation products formed during irradiation have not been identified.
Authors:
M El Sabbagh; C Galanos; L Bertók; G Füst; O Lüderitz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta microbiologica Academiae Scientiarum Hungaricae     Volume:  29     ISSN:  0001-6187     ISO Abbreviation:  Acta Microbiol Acad Sci Hung     Publication Date:  1982  
Date Detail:
Created Date:  1983-09-23     Completed Date:  1983-09-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370333     Medline TA:  Acta Microbiol Acad Sci Hung     Country:  HUNGARY    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Complement Activation
Drug Tolerance
Female
Gamma Rays
Glucosamine / analysis
Hemorrhage
Lethal Dose 50
Male
Polysaccharides, Bacterial / radiation effects*
Rabbits
Rats
Salmonella / metabolism*
Sugar Acids / analysis
Chemical
Reg. No./Substance:
0/Polysaccharides, Bacterial; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate; 3416-24-8/Glucosamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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