Document Detail


Effect of intravenous glucagon on intestinal viability after segmental mesenteric ischemia.
MedLine Citation:
PMID:  7776469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: We have previously shown that intravenous glucagon significantly improves rat survival if given after release of superior mesenteric artery occlusion. The purpose of this study was to isolate the effects of glucagon on the intestine by creating a rat model of segmental mesenteric ischemia that avoids the systemic shock associated with total superior mesenteric artery occlusion and reperfusion.
METHODS: In 18 anesthetized Sprague-Dawley rats, an 8 cm segment of midileum was made totally ischemic for 110 minutes by occluding its vascular arcades and the bowel ends with microvascular clamps. Control animals (n = 8) received normal saline solution (10 ml/kg/hr intravenously) during ischemia and for 2 hours after declamping. Ten animals also received glucagon (1 microgram/kg/min intravenously) during 2 hours after declamping. After a 24-hour recovery, the ischemic bowel segment, plus nonischemic proximal ileum, was examined histologically by computerized planimetry to measure wall thickness (muscularis, mucosa, and transmural), percent epithelial coverage, and villar surface ratio (villar surface length/bowel circumference). All observations were blinded. Comparisons were made by Student's t test.
RESULTS: Compared with nonischemic ileum, the ischemic segment in control animals showed severe mucosal injury with a reduction in mucosal thickness to 25%, epithelial coverage to 23%, and villar surface ratio to 33% of that seen in nonischemic ileum (p < 0.01). Substantial preservation of ileal mucosal viability was seen in glucagon-treated animals. Mucosal thickness and epithelial coverage were twice as well preserved in glucagon-treated rats compared with control rats (p < 0.05), and villar surface ratio was also increased significantly (p < 0.05). The muscularis was not significantly injured in this model.
CONCLUSIONS: By use of quantitative histologic measurements, we found that intravenous glucagon significantly improved ileal mucosal viability when given early during reperfusion after segmental ischemia. The mechanism of this effect and its potential clinical application merit further study.
Authors:
S P Gangadharan; R J Wagner; J L Cronenwett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of vascular surgery     Volume:  21     ISSN:  0741-5214     ISO Abbreviation:  J. Vasc. Surg.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-07-12     Completed Date:  1995-07-12     Revised Date:  2012-10-03    
Medline Journal Info:
Nlm Unique ID:  8407742     Medline TA:  J Vasc Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  900-7; discussion 907-8     Citation Subset:  IM    
Affiliation:
Section of Vascular Surgery, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Glucagon / administration & dosage*
Ileum / blood supply,  pathology*
Infusions, Intravenous
Intestinal Mucosa / blood supply,  pathology
Ischemia / pathology*
Male
Mesenteric Vascular Occlusion / pathology*
Rats
Rats, Sprague-Dawley
Tissue Survival / drug effects
Chemical
Reg. No./Substance:
9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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