Document Detail


Effect of intraperitoneal neutrophils induced by OK432 on malignant ascites.
MedLine Citation:
PMID:  15622466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To evaluate the efficacy of a streptococcal preparation, OK432, on malignant ascites in mice. METHODS: PC-C203U (PC203) is a preparation of another strain of the streptococcal family, with the lowest antineoplastic action. To examine the survival curves of mice after the inoculation of BAMC-1 tumor cells, we gave intraperitoneal OK432, PC203, or saline as a control. Intraperitonal neutrophils were counted by cytospin, and interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and macrophage inflammatory protein (MIP)-1alpha were measured by enzyme-linked immunosorbent assay, and 8 h after the administration of OK432, PC203, and saline. Using electron microscopy, we examined the greater omental milky spots, where the in situ proliferation of neutrophils or macrophages takes place. RESULTS: The OK432 group had the best survival and the control group the worst. The ratio of intraperitoneal neutrophils to BAMC-1 was highest in the OK432 group and lowest in the control group. Quantitative IL-1beta, IL-6 and MIP-1alpha levels were correlated closely with survival. Electron microscopic examination of the milky spots revealed massive proliferation of neutrophils in the OK432 group, but not in the PC203 or control groups. CONCLUSION: OK432 effectively activated intraperitoneal neutrophils and a series of immunological chain reactions through an increase in IL-1beta, IL-6, and MIP-1alpha levels. Milky spots could have important antitumor effects in terms of the spread of neutrophils.
Authors:
Yoshinori Hosoya; Eiji Kobayashi; Toshihiro Takizawa; Akio Fujimura; Yoshikazu Yasuda; Hideo Nagai; Kyotaro Kanazawa
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Surgery today     Volume:  35     ISSN:  0941-1291     ISO Abbreviation:  Surg. Today     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-28     Completed Date:  2005-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204360     Medline TA:  Surg Today     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  60-6     Citation Subset:  IM    
Affiliation:
Department of Surgery, Jichi Medical School, 3311-1 Minamikawachi, Tochigi, 329-0498, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascites / pathology*
Cell Line, Tumor
Cytokines / blood
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Mice
Mice, Inbred BALB C
Neoplasm Transplantation / mortality*
Neutrophils / drug effects,  physiology*
Peritoneal Neoplasms / drug therapy,  pathology*
Picibanil / pharmacology*
Probability
Random Allocation
Reference Values
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Cytokines; 39325-01-4/Picibanil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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