| Effect of inhibitor and activator of ghrelin receptor (GHS-R1a) on porcine ovarian granulosa cell functions. | |
| | |
MedLine Citation:
|
PMID: 21600209 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
It was previously shown, that ghrelin and its agonistic analogue, ghrelin 1-18, can be a stimulator of ovarian cell functions (promoter of proliferation, inhibitor of apoptosis and stimulator of hormones release). The aim of our studies was to compare the action of two ghrelin analogues - ghrelin 1-18, activator of ghrelin receptors (GHS-R1a), and (d-Lys3)-GHRP-6, its inhibitor, on porcine ovarian granulosa cell functions. Effects of (d-Lys3)-GHRP-6 added at doses of 0, 1, 10 or 100ng/ml on the expression of markers of proliferation (PCNA, cyclin B1, MAPK/ERK1,2), apoptosis (bax, p53, caspase 3) and release of steroid hormones (progesterone, testosterone, estradiol) were examined. In addition, some effect of ghrelin 1-8 on some of these parameters (expression of MAPK/ERK1,2, bax, p53) were verified. It was shown, that (d-Lys3)-GHRP-6 promotes all markers of granulosa cell proliferation, inhibits all markers of apoptosis and stimulates the release of all three steroid hormones. Similar effects of (d-Lys3)-GHRP-6 (inhibitor of GHS-R1a) and ghrelin 1-18 (its stimulator) suggest that the examined effects of these substances on porcine ovaries are not mediated by GHS-R1a. Both chemical analogues could be potentially useful for stimulation of reproductive processes, at least in in vitro conditions. |
| | |
Authors:
|
Alexander V Sirotkin; Monika Meszarošová; Roland Grossmann; Andrej Benčo; Francisco Valenzuela |
Related Documents
:
|
7198259 - Maternal-neonatal interactions and human breast milk. 20412849 - Role of amphotericin b upon enhancement of protective immunity elicited by oral adminis... 9498789 - A helminth-induced mucosal th2 response alters nonresponsiveness to oral administration... 20594129 - Mucosal t cells in gut homeostasis and inflammation. 21468589 - Intestinal inflammation and tissue injury in response to heat stress and cooling treatm... 17206709 - Oral biopsies from patients with orofacial granulomatosis with histology resembling cro... 20700059 - A role for syndecan-1 and claudin-2 in microbial translocation during hiv-1 infection. 16365459 - Innate stimuli accentuate end-organ damage by nephrotoxic antibodies via fc receptor an... 17477819 - Innate interferon response in macrophage and epithelial cells infected with wild-type c... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-5-10 |
Journal Detail:
|
Title: General and comparative endocrinology Volume: - ISSN: 1095-6840 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-5-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
|
Institute of Genetics and Reproduction, Animal Production Research Centre Nitra, 951 41 Lužianky near Nitra, Slovakia; Dept. of Zoology and Anthropology, Constantine the Philosopher University, 949 74 Nitra, Slovakia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Estrogenic regulation of dopaminergic neurons in the opportunistically breeding zebra finch.
Next Document: Cloning and expression of the translocator protein (18kDa), voltage-dependent anion channel, and dia...