| Effect of the inhibitor of NO synthase, NG-nitro-L-arginine methyl ester, on histamine-induced bronchospasm in the rabbit. | |
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MedLine Citation:
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PMID: 9381949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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New Zealand male rabbits were anaesthetized with thiopental, tracheotomized, curarized by vecuronium bromide and mechanically ventilated. Six rabbits received L-NAME 10 mg kg-1 i.v., six rabbits L-NAME 15 mg kg-1 iv, and six rabbits received saline i.v. (controls), 5 min before a histamine aerosol (2% solution during 5 min). Six others rabbits received an injection of L-NAME 15 mg kg-1 iv, 5 min before the histamine aerosol, followed by an infusion of L-arginine over a 60- min period. Total respiratory resistance (Rrs) and elastance (Ers) were derived by least square analysis of the relationship between tracheal pressure and flow, and computed every minute before and over a 1-h period after the histamine aerosol. Oxygen free radicals (OFR) were measured with a luminometer, in microsomes from lung homogenates at the end of the experiment. Compared with the histamine response of the control group, the Rrs response in the L-NAME 10 group was slightly less, while Ers changes were the same in the two groups. In contrast, L-NAME 15 was responsible for an increased Rrs response, the difference being significant (P < 0.05) only between 15 and 40 min after the aerosol (+114% vs. +85% in controls at the 20th min). The increase in Ers with L-NAME 15 was stronger and significantly larger (+71% vs. +42% in controls at the 20th min after the histamine aerosol, P < 0.001). The relatively greater effect of L-NAME on Ers than on Rrs suggests that NO predominantly modulates the response to histamine of the peripheral lung rather than that of the large airways. Furthermore, the effect of L-NAME on Rrs was completely abolished by L-arginine, while its effect on Ers was only partially reversed. This suggests that the changes in Ers are partly related to a hardly reversible phenomenon. Possibly, the mechanical changes are linked with the rise of OFR in the lung parenchyma, which were significantly higher in the L-NAME 15 group compared to the control group (P < 0.05). |
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Authors:
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P Dewachter; M Vassiliou; C G Saunier; D Hartemann; R Peslin; M C Laxenaire |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta physiologica Scandinavica Volume: 161 ISSN: 0001-6772 ISO Abbreviation: Acta Physiol. Scand. Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1997-11-07 Completed Date: 1997-11-07 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370362 Medline TA: Acta Physiol Scand Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 47-53 Citation Subset: IM |
Affiliation:
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Department d'Anesthésiologie, Hôpital central, Nancy, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / drug effects Arginine / pharmacology Blood Pressure / drug effects Bronchial Spasm / chemically induced, drug therapy* Enzyme Inhibitors / pharmacology* Free Radicals Histamine Injections, Intravenous Lung / metabolism Male Microsomes / metabolism NG-Nitroarginine Methyl Ester / pharmacology* Nitric Oxide Synthase / antagonists & inhibitors* Oxygen / blood Rabbits Reactive Oxygen Species Vascular Resistance / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Free Radicals; 0/Reactive Oxygen Species; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-45-6/Histamine; 74-79-3/Arginine; 7782-44-7/Oxygen; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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