Document Detail

Effect of inhaled leukotriene D4 on airway eosinophilia and airway hyperresponsiveness in asthmatic subjects.
MedLine Citation:
PMID:  10228127     Owner:  NLM     Status:  MEDLINE    
Inhaled cysteinyl leukotrienes may cause recruitment of eosinophils into asthmatic airways. We compared the effects of inhaled leukotriene D4 (LTD4), methacholine, and allergen on airway eosinophils in 10 nonsmoking, atopic, mildly asthmatic subjects in a double-blind, diluent-controlled, randomized crossover study. Concentrations of LTD4, methacholine, and allergen resulting in a 30% decrease in FEV1, and diluent controls (ethanol and saline), were inhaled with at least 7 d between challenges. Spirometry was conducted for 4 h after inhalation challenge, and airway hyperresponsiveness (AHR) to methacholine was measured before and 24 h after challenge. Sputum was induced before and 4 h, 7 h, and 24 h after challenge. The maximum decrease in FEV1 was 31.4 +/- 1.8% with LTD4, 39.4 +/- 2.8% with methacholine, and 30.1 +/- 3.4% with allergen. AHR to methacholine, at the provocative concentration causing a 20% decrease in FEV1 (PC20), was enhanced 24 h after allergen challenge, but remained unchanged 24 h after LTD4 and methacholine (p > 0.05). The percentage of eosinophils in sputum was increased after inhalation of allergen at 7 h and 24 h (p = 0.003), but not after LTD4 or methacholine (p = 0.70). We demonstrated that neither inhalation of LTD4 nor of methacholine at concentrations causing submaximal bronchoconstriction increases the number of sputum eosinophils in the airways of mildly asthmatic subjects. However, LTD4 may still be an important cofactor for eosinophil recruitment in asthma.
A Mulder; G M Gauvreau; R M Watson; P M O'byrne
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  159     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-06-14     Completed Date:  1999-06-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1562-7     Citation Subset:  AIM; IM    
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
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MeSH Terms
Administration, Inhalation
Allergens / immunology
Asthma / immunology,  physiopathology*
Bronchoconstrictor Agents
Cross-Over Studies
Double-Blind Method
Eosinophilia / chemically induced*,  pathology
Forced Expiratory Volume / physiology
Leukotriene D4 / administration & dosage*
Methacholine Chloride
Respiratory Hypersensitivity / chemically induced*,  physiopathology
Respiratory System / pathology*
Sputum / cytology
Reg. No./Substance:
0/Allergens; 0/Bronchoconstrictor Agents; 62-51-1/Methacholine Chloride; 73836-78-9/Leukotriene D4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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