Document Detail

Effect of ingestion of excess methionine diet on aging of erythrocytes in mice.
MedLine Citation:
PMID:  1629786     Owner:  NLM     Status:  MEDLINE    
Accelerated senescence prone mice and resistant mice (SAM-P/1 and SAM-R/1) were fed 10% casein diet or that supplemented with 2.5% methionine (Met) diet for 4 weeks. Erythrocytes withdrawn from animals of each group were separated by density gradient centrifugation into four fractions of different cell density, that is, different age of erythrocytes, and were analyzed for the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and the level of oxidized protein. Excess Met decreased the specific activity of GSH-Px in each fraction and the whole population of erythrocytes in SAM-P/1 but not in SAM-R/1. The changes in SOD activities were least significant among the erythrocyte fractions of every dietary group. The oxidized protein level in cell lysate increased with the increase of cell density in both strains of animals. These results were interpreted as indicating that excess Met had a positive effect on the erythrocytes senescence, and suggested that the activity of GSH-Px may be a useful enzymatic marker for the age of erythrocytes.
P He; K Yasumoto
Related Documents :
25331786 - Effects of dietary interventions on incidence and progression of ckd.
2127976 - Histopathological and biochemical changes associated with selenium and vitamin e defici...
19577296 - Carcinogenic effect of arsenate in c57bl/6j/han mice and its modulation by different di...
10466196 - Functional indicators of iodine and selenium status.
7547826 - Dietary change after smoking cessation: a prospective study.
10917926 - Dietary intake and bioavailability of polyphenols.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of nutritional science and vitaminology     Volume:  38     ISSN:  0301-4800     ISO Abbreviation:  J. Nutr. Sci. Vitaminol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-08-14     Completed Date:  1992-08-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0402640     Medline TA:  J Nutr Sci Vitaminol (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  57-68     Citation Subset:  IM    
Research Institute for Food Science, Kyoto University, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Proteins / analysis
Chemical Fractionation
Erythrocyte Aging / drug effects*
Erythrocytes / enzymology
Hemoglobins / analysis
Methionine / administration & dosage,  adverse effects*
Reg. No./Substance:
0/Blood Proteins; 0/Hemoglobins; 63-68-3/Methionine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Role of dihydropyrimidine dehydrogenase in the uridine nucleotide metabolism in the rat liver.
Next Document:  Lowering and delaying actions of bovine bile on plasma ethanol levels in rats.