| Effect of inducers on the production of 5-aminolevulinic acid by recombinant Escherichia coli. | |
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MedLine Citation:
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PMID: 16707333 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aminolevulinic acid (ALA) was produced by recombinant Escherichia coli BL21(DE3) (pET28-A.R-hemA) harboring the ALA synthase gene (hemA) from Agrobacterium radiobacter zju-0121. The effects of inducers on the ALA synthase activity and ALA productivity were evaluated. The results indicated that a low isopropyl-beta-D-thiogalactoside (IPTG) concentration (0.05 mmol/L) was favorable for high expression of ALA synthase, which resulted in higher ALA productivity. For metabolic engineering applications, lactose was a better substitute of IPTG for active enzyme expression. When lactose concentration was 5 mmol/L, the specific ALA synthase activity and ALA productivity reached 16.7 nmol/(min . mg of protein) and 1.15 g/L, respectively, which were about 15% and 43% higher than those induced by IPTG. |
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Authors:
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Liu Xiaoxia; Lin Jianping; Cen Peilin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Preparative biochemistry & biotechnology Volume: 36 ISSN: 1082-6068 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-05-18 Completed Date: 2007-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 223-33 Citation Subset: IM |
Affiliation:
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Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou, P. R. China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5-Aminolevulinate Synthetase
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genetics,
metabolism Aminolevulinic Acid / metabolism* Enzyme Induction / drug effects Escherichia coli / drug effects*, genetics, metabolism* Isopropyl Thiogalactoside / pharmacology Lactose / metabolism, pharmacology |
| Chemical | |
Reg. No./Substance:
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106-60-5/Aminolevulinic Acid; 367-93-1/Isopropyl Thiogalactoside; 63-42-3/Lactose; EC 2.3.1.37/5-Aminolevulinate Synthetase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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