Document Detail


Effect of incubation at overwintering temperatures on the replication of West Nile Virus in New York Culex pipiens (Diptera: Culicidae).
MedLine Citation:
PMID:  11372976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the effect of simulated overwintering temperatures on West Nile (WN) virus replication in Culex pipiens L. derived from mosquitoes collected during the autumn 1999 WN epizootic in New York. The WN virus was a strain isolated from a dead crow also collected during this outbreak. Virus was recovered from most mosquitoes held exclusively at 26 degres C. In contrast, none of the mosquitoes held exclusively at the lower temperatures had detectable infections. When mosquitoes were transferred to 26 degrees C after being held at 10 degrees C for 21-42 d, infection and dissemination rates increased with increased incubation at 26 degrees C. Future studies involving the attempted isolation of WN virus from overwintering mosquitoes may benefit from holding the mosquitoes at 26 degrees C before testing for infectious virus.
Authors:
D J Dohm; M J Turell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical entomology     Volume:  38     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-24     Completed Date:  2001-07-05     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  462-4     Citation Subset:  IM    
Affiliation:
Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702-5011, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Culex / virology*
New York
Seasons
Temperature
Virus Replication*
West Nile virus / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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