Document Detail

Effect of increased uptake of plasma fatty acids by the liver on lipid metabolism in the hepatocellular nuclei.
MedLine Citation:
PMID:  9250605     Owner:  NLM     Status:  MEDLINE    
The nucleus contains different lipids. The aim of the present study was to examine whether increased uptake of free fatty acids by the liver affects lipid metabolism in the hepatocellular nuclei. The experiments were carried out on three groups of Wistar rats: I - male, control; II - male, heparin-treated, and III - female. [14C]-palmitic acid suspended in rat donor serum was administered intravenously 5 and 30 min before tissue samples were taken. Lipids were extracted from isolated liver nuclei and separated into different fractions (phospholipids - PH, monoacylglycerols - MG, diacylglycerols - DG, cholesterol - CH, free fatty acids - FFA, triacylglycerols - TG and cholesterol esters - CHE). It was found that 5 min after administration of the label all isolated nuclear lipid fractions were radioactive. Most of the radioactivity was located in the fraction of PH, TG and FFA. Elevation in the plasma FFA concentration (heparin-treated group) resulted in increased incorporation of [14C]-palmitic acid into the nuclear lipids and changes in its distribution. In the female rats the radioactivity of nuclear lipids was higher than in the male-controls. There were also differences in the percentage distribution of the radioactivity in different lipid fractions between the two groups. The concentration of PH and TG in the nuclei increased only in the heparin-treated but not in the female rats. However, specific activity of the nuclear PH and TG increased in with both groups compared to the male-control group. It is concluded that (a) the blood-borne FFA rapidly enter the nuclear lipid pool and (b) increased uptake of the plasma-borne FFA by the liver affects the nuclear lipid metabolism.
R Bucki; M Zendzian-Piotrowska; A Nawrocki; J Górski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  57     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-01     Completed Date:  1997-10-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  27-31     Citation Subset:  IM    
Department of Physiology, Medical Academy of Biatystok, Poland.
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MeSH Terms
Carbon Radioisotopes
Cell Nucleus / metabolism*
Fatty Acids / blood*,  metabolism
Fatty Acids, Nonesterified / blood,  metabolism
Heparin / pharmacology*
Lipid Metabolism*
Liver / metabolism*,  ultrastructure*
Palmitic Acid / metabolism
Phospholipids / metabolism
Rats, Wistar
Triglycerides / metabolism
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Phospholipids; 0/Triglycerides; 57-10-3/Palmitic Acid; 9005-49-6/Heparin

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