Document Detail

Effect of in vivo injection of cholera and pertussis toxin on glucose transport in rat skeletal muscle.
MedLine Citation:
PMID:  9038845     Owner:  NLM     Status:  MEDLINE    
Cholera toxin (CTX) and pertussis toxin (PTX) were examined for their ability to inhibit glucose transport in perfused skeletal muscle. Twenty-five hours after an intravenous injection of CTX, basal transport was decreased approximately 30%, and insulin- and contraction-stimulated transport was reduced at least 86 and 49%, respectively, in both the soleus and red and white gastrocnemius muscles. In contrast, PTX treatment was much less efficient. Impairment of glucose transport appeared to develop 10-15 h after CTX administration, which coincided with development of hyperglycemia despite hyperinsulinimia, increased plasma free fatty acid levels, increased adenosine 3',5'-cyclic monophosphate (cAMP) concentrations in muscle, but no difference in plasma catecholamines. Twenty-five hours after CTX treatment, GLUT-4 protein in both soleus and red gastrocnemius muscles was decreased, whereas no change in GLUT-1 protein content was found. In contrast, GLUT-4 mRNA was unchanged, but transcripts for GLUT-1 were increased > or = 150% in all three muscles from CTX-treated rats. The findings suggest that CTX via increased cAMP impairs basal as well as insulin- and contraction-stimulated muscle glucose transport, at least in part from a decrease in intramuscular GLUT-4 protein.
T Ploug; X Han; L N Petersen; H Galbo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  272     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-28     Completed Date:  1997-03-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E7-17     Citation Subset:  IM    
Department of Medical Physiology, Panum Institute, University of Copenhagen, Denmark.
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MeSH Terms
Biological Transport / drug effects
Catecholamines / metabolism
Cholera Toxin / pharmacology*
Cyclic AMP / metabolism
Glucose / metabolism*
Glucose Transporter Type 1
Glucose Transporter Type 4
Injections, Intravenous
Insulin / pharmacology
Monosaccharide Transport Proteins / genetics,  metabolism
Muscle Contraction / physiology
Muscle Proteins*
Muscle, Skeletal / metabolism*
Pertussis Toxin*
RNA, Messenger / metabolism
Rats, Wistar
Virulence Factors, Bordetella / pharmacology*
Reg. No./Substance:
0/Catecholamines; 0/Glucose Transporter Type 1; 0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/RNA, Messenger; 0/Slc2a1 protein, rat; 0/Slc2a4 protein, rat; 0/Virulence Factors, Bordetella; 11061-68-0/Insulin; 50-99-7/Glucose; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin; EC Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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