| Effect in vitro of apolipoprotein A-V on the structure and functions of recombinant high density lipoprotein. | |
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MedLine Citation:
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PMID: 20869952 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The neighboring position of apolipoprotein A-I (apoA-I) and apolipoprotein A-V (apoA-V) gene and the modulation of apoA-V on the concentrations, size and maturation of high density lipoprotein (HDL) may indicate a special relationship between apoA-V and HDL. To assess the effects of apoA-V on HDL structure and related functions in vitro, a series of recombinant HDL (rHDL) were synthesized in vitro with various mass ratios of recombinant apoA-I: apoA-V. An increase in apoA-V in rHDL resulted in enhanced lipid-binding ability, increased phospholipid content and larger particle size. Furthermore, the lipid-free and lipid-bound apoA-V in rHDL showed antioxidant capacity against low density lipoprotein (LDL) in vitro. In THP-1 derived macrophages, apoA-V of rHDL was shown to have no influence on the uptake of oxidized LDL (oxLDL) and intracellular lipid accumulation. Thus, the addition of apoA-V to rHDL resulted in changes in several rHDL properties, including increased lipid-binding ability, phospholipid content, particle size and antioxidant capacity. These alterations may explain the modulation of apoA-V on HDL in vivo and the beneficial functions of apoA-V on atherosclerosis. |
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Authors:
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Xinbo Zhang; Jian Wang; Baosheng Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-24 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 401 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-25 Completed Date: 2010-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 473-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apolipoproteins A
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metabolism* Atherosclerosis / metabolism* Cell Line Humans Lipoproteins, HDL / chemistry, genetics, metabolism* Lipoproteins, LDL / chemistry, genetics, metabolism Macrophages / metabolism Oxidation-Reduction Protein Conformation Protein Transport Recombinant Proteins / chemistry, genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/APOA5 protein, human; 0/Apolipoproteins A; 0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 0/Recombinant Proteins; 0/oxidized low density lipoprotein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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