| Effect of hypoxia mimetic cobalt chloride on the expression of extracellular-superoxide dismutase in retinal pericytes. | |
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MedLine Citation:
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PMID: 21804221 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The initial clinical stage of diabetic retinopathy (DR) is characterized by the development of intraretinal microvascular abnormalities. The increased formation of reactive oxygen species (ROS) is thought to be a key event in the pathogenesis of DR. Extracellular-superoxide dismutase (EC-SOD) is an anti-inflammatory enzyme that is distributed mainly in vascular cells and protects cells from ROS by scavenging superoxide anion. Treatment with cobalt chloride (CoCl(2)) decreased the expression of EC-SOD but not other SOD isozymes in pericytes accompanied with an increase of intracellular ROS production. Pre-treatment with N-acetylcysteine (NAC) significantly suppressed the ROS production and down-regulation of EC-SOD. We observed the activation of caspase-3 and DNA fragmentation as signs of apoptotic process by CoCl(2) treatment. In addition, these phenomena were significantly inhibited by pre-treatment with NAC. EC-SOD enhancer 4-phenyl butyric acid also suppressed the caspase-3 activation. It is known that the presence of a high level of EC-SOD throughout the vessel walls might have an important protective role against superoxide in the vascular system. The decrease in EC-SOD expression accompanied with elevation of ROS level in pericytes under hypoxia might induce and/or promote the ROS-triggered apoptosis of pericytes and the development of pathogenesis in DR. |
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Authors:
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Tetsuo Adachi; Kazunari Aida; Hiroko Nishihara; Tetsuro Kamiya; Hirokazu Hara |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 34 ISSN: 1347-5215 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-08-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 1297-300 Citation Subset: IM |
Affiliation:
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Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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