Document Detail


Effect of hypoxia on cerebral metabolites measured by proton nuclear magnetic resonance spectroscopy in rats.
MedLine Citation:
PMID:  1846248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proton nuclear magnetic resonance spectroscopy is a unique method to monitor noninvasively the concentrations of cerebral metabolites. N-Acetyl-L-aspartate, the concentration of which is assumed to be stable during hypoxia, has been used to form ratios with lactate. To determine the stability of the signal from N-acetyl-L-aspartate, we used a model of graded hypoxia in rats to monitor the percentage changes from baseline of the peak heights for lactate, lipids, and N-acetyl-L-aspartate. Anesthetized adult rats were exposed sequentially to 15% and 10% O2 while proton nuclear magnetic resonance spectra were collected with a surface coil in a 7-T 89-mm-bore spectrometer. Brain lactate concentration was either increased by feeding or infusion of glucose (n = 9) or lowered by fasting (n = 7). After death the brains were removed and frozen, and the water- and lipid-soluble compounds were extracted to identify the origin of the signals. We analyzed the data both as the percentage change from baseline for heights of the lactate (1.33 ppm), lipids (1.5 ppm), and N-acetyl-L-aspartate (2.02 ppm) peaks and as the ratios of heights of the 1.33 and 2.02 and the 1.5 and 2.02 ppm peaks. Both hypoxic episodes caused a 45% decrease from baseline in the 2.02 ppm peak. During the second hypoxic episode, the 1.33:2.02 ppm peak height ratio increased significantly in hyperglycemic rats (p less than 0.05) but was unchanged in hypoglycemic rats.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
G A Rosenberg; J White; C Gasparovic; E A Crisostomo; R H Griffey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  22     ISSN:  0039-2499     ISO Abbreviation:  Stroke     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-02-19     Completed Date:  1991-02-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  73-9     Citation Subset:  IM    
Affiliation:
Neurology Service, Veterans Affairs Medical Center, Albuquerque, N.M.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia / metabolism*
Aspartic Acid / analogs & derivatives,  metabolism
Brain / metabolism*
Hyperglycemia / metabolism
Hypoglycemia / metabolism
Lactates / metabolism
Lactic Acid
Magnetic Resonance Spectroscopy*
Protons
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
R01 NS21169-04/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Lactates; 0/Protons; 50-21-5/Lactic Acid; 56-84-8/Aspartic Acid; 997-55-7/N-acetylaspartate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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