Document Detail

Effect of hypoxemia on tissue glycogen content and glycolytic enzyme activities in fetal sheep.
MedLine Citation:
PMID:  9038997     Owner:  NLM     Status:  MEDLINE    
We have tested the hypothesis that prolonged fetal hypoxemia causes a reduction in glycogenolytic enzyme activities and/or a depletion of fetal glycogen stores. We compared the effects of short (4 h) and prolonged (24 h) periods of reduced maternal uterine blood flow (RUBF) on glycogen content and on the activities of glucose-6-phosphatase (G-6-Pase), glycogen phosphorylase (GPase), and glycogen synthase (GSase) in selected fetal tissues. RUBF was reduced in 10 pregnant sheep at 135 days of gestation (term approximately 146 days) for either 4 h (n = 5) or 24 h (n = 5); 5 other fetuses were used as controls. During RUBF, fetal SaC2 was decreased from 61.6 +/- 3.9 to 22.0 +/- 1.4% at 4 h and to 26.7 +/- 1.2% at 24 h. Hepatic glycogen content was significantly reduced at 4 h of RUBF, but was not reduced further at 24 h. Fetal liver GPase (active and total enzyme activity) and G-6-Pase activities were reduced at 4 h of RUBF but tended to return toward control values at 24 h. Similarly, hepatic GSase activity tended to decrease at 4 h of RUBF, although the reduction was not quite significant (P = 0.08). We conclude that RUBF causes a reduction of fetal glycogen stores and a reduction in G-6-Pase and GPase activity at 4 h. Fetal tissue glycogen contents were not reduced further at 24 h, compared with 4 h of RUBF, which indicates that fetal glycogenolysis is reduced during this time, probably because of the inhibition of GPase and G-6-Pase. It is not known why the activities of these enzymes are reduced during prolonged RUBF, when circulating epinephrine and norepinephrine concentrations are high.
L L Stratford; S B Hooper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  272     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-31     Completed Date:  1997-03-31     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R103-10     Citation Subset:  IM    
Department of Physiology, Monash University, Clayton, Victoria, Australia.
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MeSH Terms
Anoxia / enzymology*,  metabolism*
Blood Glucose / analysis
Cyclic AMP / metabolism
Fetal Blood
Fetus / metabolism*
Gases / blood
Glucose-6-Phosphatase / metabolism
Glycogen / metabolism*
Glycogen Synthase / metabolism
Hydrogen-Ion Concentration
Lactic Acid / blood
Liver / embryology,  metabolism
Norepinephrine / blood
Osmolar Concentration
Oxygen / blood
Phosphorylases / metabolism
Sheep / embryology
Reg. No./Substance:
0/Blood Glucose; 0/Gases; 50-21-5/Lactic Acid; 51-41-2/Norepinephrine; 60-92-4/Cyclic AMP; 7782-44-7/Oxygen; 9005-79-2/Glycogen; EC 2.4.1.-/Phosphorylases; EC Synthase; EC

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