Document Detail

Effect of hypothermia on cellular membrane function during low-flow extracorporeal circulation.
MedLine Citation:
PMID:  3616909     Owner:  NLM     Status:  MEDLINE    
The use of systemic hypothermia is known to allow recovery from potentially lethal states of profound hypoperfusion or total circulatory arrest. While the cellular alterations accompanying states of decreased perfusion in skeletal muscle are well defined, little is known regarding the impact of coexistent hypothermia. To investigate this issue, nine dogs were placed on total cardiopulmonary bypass (CPB) and perfused in nonpulsatile fashion. The following flow and temperature parameters were used in three different perfusion models: 3.5 L/min/m2 at 23 degrees C (group A, n = 3), 1.6 L/min/m2 at 37 degrees C (group B, n = 3), and 1.6 L/min/m2 at 23 degrees C (group C, n = 3). Assessment of cellular function in a hind limb adductor muscle by measurement of resting transmembrane potential difference (Em) and determination of tissue electrolyte distribution in a biopsy specimen was performed in the control state and again after 60 minutes of total CPB. Low-flow/hypothermic CPB (group C) was associated with depolarization of resting Em to -63.3 +/- 3.2 mV from a control value of -87.0 +/- 1.3 mV (p less than 0.05), an increase in the calculated intracellular Na ([Na]i) to 16.4 +/- 4.0 mEq/L from a control value of 7.6 +/- 1.4 mEq/L (p less than 0.05), and an increase in the ratio of the selective membrane permeabilities of Na+ to K+ (pNa/pK), to 0.067 +/- 0.013 from a control value of 0.013 +/- 0.002 (p less than 0.05). In contrast, resting Em was maintained at -86.4 +/- 6.1 mv during normal-flow/hypothermic CPB (group A), while low-flow/normothermic CPB (group B) produced an intermediate depolarization to -75.2 +/- 3.0 mV (p less than 0.05). Neither [Na]i or pNa/pK was altered significantly in group A or group B dogs. These data characterize a physiologic alteration in the cellular membrane function of skeletal muscle during low-flow/hypothermic CPB, which is similar in many respects to that accompanying hemorrhagic shock. This suggests that during periods of profound hypothermia certain flow-related derangements in skeletal muscle are not obviated and may be exacerbated.
G A Fantini; B J Zadeh; J Chiao; K H Krieger; O W Isom; G T Shires
Related Documents :
2558099 - Muscle malonyl-coa decreases during exercise.
14717029 - Using weights in abdominal exercises: electromyography response of the rectus abdominis...
16457979 - Aging alters macrophage properties in human skeletal muscle both at rest and in respons...
15741839 - Reduced heterogeneity of muscle deoxygenation during heavy bicycle exercise.
19359609 - Ampk activation is fiber type specific in human skeletal muscle: effects of exercise an...
2824119 - Predictable adaptations by skeletal muscle mitochondria to different exercise training ...
8281369 - Peroxide plasma level in patients with coronary heart disease as a possible indicator o...
17102979 - Effects of adaptive exercise on apoptosis in cells of rat renal tubuli.
16516509 - Exercise-induced alterations in plasma concentrations of ghrelin, adiponectin, leptin, ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Surgery     Volume:  102     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1987-08-28     Completed Date:  1987-08-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  132-9     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Membrane / physiology
Cell Membrane Permeability
Extracorporeal Circulation* / methods
Hypothermia, Induced*
Membrane Potentials
Muscles / physiology*,  ultrastructure
Potassium / metabolism
Sodium / metabolism
Grant Support
Reg. No./Substance:
7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Amaurosis fugax at downward gaze.
Next Document:  Intracellular myocardial pH measured in vivo with sustained and reperfused coronary occlusion.