| Effect of hypertonic saline resuscitation on CD4+CD25+ regulatory T cells and gammadelta T cells after hemorrhagic shock and resuscitation in relation to apoptosis and iNOS. | |
| | |
MedLine Citation:
|
PMID: 19901657 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Hemorrhagic shock and resuscitation induce immunosuppression. CD4CD25 regulatory T cells and gammadeltaT cells may affect these immunosuppressive conditions. Hypertonic saline resuscitation reduces damage to organs and apoptosis and also restores immunosuppressive condition. We investigated how hypertonic saline resuscitation affected the induction of CD4CD25 regulatory T cells and gammadeltaT cells, and their apoptosis after hemorrhagic shock and resuscitation, and its relationship to inducible nitric oxide synthase (iNOS) (nitric oxide production). METHODS: Male inbred C57BL6/J mice 8-week to 12-week-old as wild type and iNOS gene knock out (iNOS-/-), weighing 20 g to 35 g, were used. Hemorrhagic shock model of +/-40 mm Hg for 60 minutes was setup. Animals were randomly assigned to the following four resuscitation group: (1) wild HS: resuscitation with hypertonic saline (4 mL/Kg of 7.5% NaCl) and shed blood (SB), (2) wild 2LR: resuscitation with lactated Ringer's solution (two times the volume of the SB) and SB, (3) iNOS knockout HS, and (4) iNOS knockout 2LR. Untreated groups for wild and iNOS knockout mice were designated as control groups. Samples of thymus and spleen were harvested at 2 hours, 6 hours, 24 hours, and 48 hours after resuscitation. CD4CD25 regulatory T cells and gammadeltaT cells were analyzed using three-color flow cytometry. RESULTS: (1) gammadelta T cells increased earlier at 24 hours and CD4CD25 regulatory T cells increased later at 48 hours compared with controls in spleen of wild type (p < 0.01). (2) Hypertonic saline resuscitation suppressed gammadelta T cells compared with 2LR at 24 hours in iNOS knockout mice in spleen (p < 0.05). Hypertonic saline resuscitation increased apoptosis of CD4CD25 regulatory T cells at 48 hours in iNOS knockout mice in spleen (p < 0.01). (3) CD4CD25 regulatory T cells of iNOS knockout both in HS and 2LR groups at 48 hours decreased compared with wild type both in HS and 2LR groups in spleen (p < 0.01). (4) Apoptotic gammadelta T cells both in spleen and thymus in iNOS knockout mice at 48 hours increased compared with those in wild type (p < 0.05, respectively, except gammadelta T cells 2LR in spleen: p = 0.058). CONCLUSION: gammadelta T cells increased earlier at 24 hours, whereas CD4CD25 regulatory T cells increased later at 48 hours in spleen of wild type. Hypertonic saline was effective without the presence of iNOS, i.e., decreased gammadelta T cells at 24 hours and increased apoptosis of CD4CD25 regulatory T cells at 48 hours. CD4CD25 regulatory T cells at 48 hours without iNOS decreased compared with those of wild type. gammadelta T cells at 48 hours induced apoptosis under the condition without iNOS in spleen and thymus. iNOS worked as an accelerating factor for immunosuppressive condition, affected apoptosis, and immunoenhancing effect by hypertonic saline. |
| | |
Authors:
|
Yoshinori Murao; Kenji Isayama; Fukuki Saito; Akihiko Hirakawa; Toshio Nakatani |
Related Documents
:
|
20370687 - Persistent p21cip1 induction mediates g(1) cell cycle arrest by methylseleninic acid in... 20556567 - Activation of sonic hedgehog signaling pathway in s-type neuroblastoma cell lines. 12894557 - Flow cytometric evaluation of apoptosis and cell viability as a criterion of anti-tumou... 15301427 - Apoptosis of human t-cell acute lymphoblastic leukemia cells by diphenhydramine, an h1 ... 22299047 - Mir-99a and mir-99b modulate tgf-β induced epithelial to mesenchymal plasticity in nor... 3358627 - Drug sensitivity tests after cell separation on density gradients. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of trauma Volume: 67 ISSN: 1529-8809 ISO Abbreviation: J Trauma Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-11-10 Completed Date: 2009-12-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376373 Medline TA: J Trauma Country: United States |
Other Details:
|
Languages: eng Pagination: 975-82 Citation Subset: AIM; IM |
Affiliation:
|
Department of Emergency and Critical Care Medicine, Kansai Medical University, Osaka 570-8507, Japan. muraoy@takii.kmu.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / drug effects Blotting, Western Disease Models, Animal Flow Cytometry Male Mice Mice, Inbred C57BL Nitric Oxide Synthase Type II / physiology* Resuscitation Saline Solution, Hypertonic / pharmacology* Shock, Hemorrhagic / immunology, physiopathology* T-Lymphocytes, Regulatory / drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Saline Solution, Hypertonic; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunologic function in the elderly after injury--the neutrophil and innate immunity.
Next Document: Octogenarian abdominal surgical emergencies: not so grim a problem with the acute care surgery model...