| Effect of hyperthermic preconditioning on cold preserved rat portal veins. | |
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MedLine Citation:
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PMID: 12399231 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: Little information is available regarding the effect of cold storage and hyperthermic preconditioning on the contractile responses of hepatic vessels. We then studied, after cold preservation, the in vitro contractile responses of rat portal veins (RPV) isolated from normal rats or from rats previously subjected to hyperthermia. METHODS: Rats were or were not subjected to hyperthermia 24 h before the RPV isolation. Then, RPV were stored at 4 degrees C in Krebs-Henseleit bicarbonate (KHB) or University of Wisconsin solution or conserved at 20 degrees C in KHB solution. Control RPV were tested after rat sacrifice. RESULTS: The contractile responses were importantly decreased in RPV conserved at room temperature. The morphology of the vessels was altered and the heat shock protein 70 (Hsp70) protein expression disappeared. These abnormalities were prevented by cold preservation. The type of preservation solution did not interfere with the beneficial effect. Hyperthermic preconditioning increased the expression of Hsp70 protein in freshly isolated and cold preserved RPV but decreased the contractile responses. In RPV conserved at room temperature, hyperthermic preconditioning further worsened the decreased contractile response. CONCLUSIONS: Thus, hyperthermic preconditioning, which is beneficial in protecting hepatic injury following cold preservation, alters the contractile responses of RPV. |
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Authors:
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Catherine M Pastor; Jean-Louis Frossard; Gilles Mentha; Dominique Mastrangelo; Rafael Quadri; Antoine Hadengue |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of hepatology Volume: 37 ISSN: 0168-8278 ISO Abbreviation: J. Hepatol. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-25 Completed Date: 2003-06-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8503886 Medline TA: J Hepatol Country: England |
Other Details:
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Languages: eng Pagination: 640-7 Citation Subset: IM |
Copyright Information:
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Copyright 2002 European Association for the Study of the Liver |
Affiliation:
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Division d'Hépatologie et de Gastro-entérologie, Hôpital Cantonal Universitaire de Genève, Rue Micheli-du-Crest, 24, 1211 Geneva 14, Switzerland. catherine.pastor@hcuge.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chaperonin 60 / biosynthesis Cold Temperature Graft Survival HSP70 Heat-Shock Proteins / biosynthesis Hyperthermia, Induced* Ischemic Preconditioning* Male Organ Preservation Solutions Portal Vein / metabolism, transplantation* Rats Rats, Sprague-Dawley Temperature Vasoconstriction / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Chaperonin 60; 0/HSP70 Heat-Shock Proteins; 0/Organ Preservation Solutions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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