Document Detail


Effect of hyperthermia and anoxia on glucocorticoid and mineralocorticoid receptor expression in neonatal rat hippocampus.
MedLine Citation:
PMID:  19028552     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Brief periods of neonatal asphyxia are frequently observed. Within the CNS, the hippocampus is known to be particularly vulnerable to the damaging effects of hypoxia/ischaemia. The hippocampus contains the highest concentration of both mineralocorticoid (MR) and glucocorticoid (GR) receptors and the balance between MR/GR activation influences cell birth and death. MR occupation appears to promote prosurvival actions, while GR overactivation favours neurodegeneration. It has been widely recognized that core body temperature is a critical determinant of the severity of hypoxic-ischemic brain injury; indeed, hyperthermia exacerbates the degree of damage. Therefore, the aim of the present investigation was to study the effect of elevated body temperature in newborn rats under control conditions or during neonatal exposure to a critical anoxia, on changes of MR and GR mRNA expression in the rat hippocampus. 2-day-old rats were exposed to anoxia in 100% nitrogen atmosphere. Rectal temperature was kept at 33 degrees C (typical for the rat neonates), or elevated to a level typical for febrile (39 degrees C) adults. Control rats were exposed to atmospheric air under the respective thermal conditions. The changes in MR and GR mRNA expression in hippocampus were examined 24h after exposure. Our data show that hyperthermia with or without added anoxia, causes induction of MR mRNA expression in neonatal rat hippocampus without any effect on GR mRNA expression. We suggest this elevation of MR plays an important role in modulating the survival of neurons in the injured hippocampus.
Authors:
Justyna Rogalska; Peng Kang; Wendy Wotherspoon; Malcolm R Macleod; Maggie Lai
Publication Detail:
Type:  Journal Article     Date:  2008-11-18
Journal Detail:
Title:  Neuroscience letters     Volume:  450     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-19     Completed Date:  2009-03-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  196-200     Citation Subset:  IM    
Affiliation:
Department of Animal Physiology, Institute of General and Molecular Biology, N. Copernicus University, ul. Gagarina 9, 87-100 Toruń, Poland. rogal@umk.pl
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Animals, Newborn
Anoxia / pathology,  physiopathology*
Female
Gene Expression Regulation, Developmental / physiology*
Hippocampus / metabolism*
Hyperthermia, Induced / methods*
Male
RNA, Messenger / metabolism
Rats
Rats, Wistar
Receptors, Glucocorticoid / genetics,  metabolism*
Receptors, Mineralocorticoid / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Glucocorticoid; 0/Receptors, Mineralocorticoid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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