Document Detail


Effect of hyperbarically oxygenated-perfluorochemical with University of Wisconsin solution on preservation of rat small intestine using an original pressure-resistant portable apparatus.
MedLine Citation:
PMID:  17630001     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Perfluorochemicals (PFC) are chemical substances that have a higher oxygen solubility under hyperbaric oxygen (HBO) pressure. This study investigated the effect of cold HBO-PFC/University of Wisconsin (UW) solution on preservation of rat small intestinal graft. METHODS: We manufactured an air-tight, pressure-resistant tank made of stainless steel with high thermal conductivity. Rat ileal grafts were placed in a custom-made silicon-gum bag with UW solution, which was immersed in 5 atm HBO-PFC solution in the tank (Group P-5). The tank was kept at 4 degrees C. We compared the ATP concentration and mucosal permeability in Group P-5 with grafts preserved in 1 atm oxygenated-PFC/UW solution (Group P-1) and simple cold storage in UW solution (Group C). Histologic study was also performed. RESULTS: PO(2) in UW solution after 48 h preservation were 1852 +/- 37, 499 +/- 13, and 173 +/- 3 mmHg (Group P-5, P-1 and C, respectively, mean +/- SD). At 48 h of preservation, graft ATP concentration was significantly greater in Group P-5 compared to that in Group P-1 and Group C. Mucosal hyperpermeability as well as mucosal morphologic changes were also ameliorated in Group P-5. CONCLUSION: HBO-PFC can supply a greater amount of oxygen to UW solution. Indirect measures of oxygen metabolism such as ATP content and lactate production suggested improvement in maintaining graft oxygen metabolism.
Authors:
Kazunori Inuzuka; Naoki Unno; Naoto Yamamoto; Daisuke Sagara; Minoru Suzuki; Motohiro Nishiyama; Hiroyuki Konno
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Surgery     Volume:  142     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-16     Completed Date:  2007-08-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-66     Citation Subset:  AIM; IM    
Affiliation:
Second Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenosine / chemistry,  pharmacology
Adenosine Triphosphate / metabolism
Allopurinol / chemistry,  pharmacology
Animals
Atmospheric Pressure*
Carbon Dioxide / analysis
Equipment Design
Glutathione / chemistry,  pharmacology
Hydrogen-Ion Concentration
Ilium / drug effects*,  metabolism,  pathology
Insulin / chemistry,  pharmacology
Intestinal Mucosa / metabolism
Lactic Acid / analysis
Male
Organ Preservation / instrumentation*,  methods*
Organ Preservation Solutions / chemistry,  pharmacology*
Osmolar Concentration
Oxygen / administration & dosage*
Partial Pressure
Permeability / drug effects
Raffinose / chemistry,  pharmacology
Rats
Rats, Sprague-Dawley
Time Factors
Transplants
Chemical
Reg. No./Substance:
0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 124-38-9/Carbon Dioxide; 315-30-0/Allopurinol; 50-21-5/Lactic Acid; 512-69-6/Raffinose; 56-65-5/Adenosine Triphosphate; 58-61-7/Adenosine; 70-18-8/Glutathione; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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