| Effect of hyperbaric oxygen on apoptosis in neonatal hypoxia-ischemia rat model. | |
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MedLine Citation:
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PMID: 14555671 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously demonstrated that a transient exposure to hyperbaric oxygen (HBO) attenuated the neuronal injury after neonatal hypoxia-ischemia. This study was undertaken to determine whether HBO offers this neuroprotection by reducing apoptosis in injured brain tissue. Seven-day-old rat pups were subjected to unilateral carotid artery ligation followed by 2 h of hypoxia (8% oxygen). Apoptotic cell death was examined in the injured cortex and hippocampus tissue. Caspase-3 expression and activity increased at 18 and 24 h after the hypoxia-ischemia insult. At 18-48 h, poly(ADP-ribose) polymerase (PARP) cleavage occurred, which reduced the band at 116 kDa and enhanced the band at 85 kDa. There was a time-dependent increase in the number of terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL)-positive cells. A single HBO treatment (100% oxygen, 3 ATA for 1 h) 1 h after hypoxia reduced the enhanced caspase-3 expression and activity, attenuated the PARP cleavage, and decreased the number of TUNEL-positive cells observed in the cortex and hippocampus. These results suggest that the neuroprotective effect of HBO is at least partially mediated by the reduction of apoptosis. |
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Authors:
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John W Calvert; Changman Zhou; Anil Nanda; John H Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 95 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-10-13 Completed Date: 2004-06-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 2072-80 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Louisiana State University Health Sciences Center-Shreveport, Shreveport, Lousiana 71130, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Caspase 3 Caspases / metabolism DNA Fragmentation* Disease Models, Animal Female Hyperbaric Oxygenation* Hypoxia-Ischemia, Brain / metabolism, pathology*, therapy* In Situ Nick-End Labeling Male Nerve Degeneration / metabolism, pathology, therapy Organ Size Poly(ADP-ribose) Polymerases / metabolism Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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NS-45694/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
| Comments/Corrections | |
Erratum In:
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J Appl Physiol. 2004 Jan;96(1):405 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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