Document Detail

Effect of hydroxy-vitamin D3 derivatives on 45Ca release from rat fetal bones in vitro.
MedLine Citation:
PMID:  401017     Owner:  NLM     Status:  MEDLINE    
Several hydroxy vitamin D3 (OH-D3) derivatives were tested for biological activity by measuring 45Ca release from prelabeled rat fetal bones, in vitro. In a 72 h continuous culture, 1 alpha,25-(OH)2-D3 produced a significant effect at 10 pg/ml. A similar effect was produced by 50 ng/ml of either 25-OH-D3 or 5,6-trans-25-OH-D3, and by 500 pg of 3 deoxy-1 alpha,25-(OH)2-D3. 24R,25-(OH)2-D3 was more active than 24S,25-(OH)2-D3, and the R isomer had activity that more closely resembled the biosynthetic compound. 3-deoxy-1 alpha-OH-D3 was inactive at a concentration of up to 1 microgram/ml. Using a 24 h preincubation period with no added vitamin D3 derivative, a steep dose-response curve could be obtained with 1 alpha,25-(OH)2-D3 over a range of 2-10 pg/ml during a subsequent 96 h incubation period, and 1 alpha,25-(OH)2-D3 was found to have about 5000 times the activity of 25-OH-D3.
A Mahgoub; H Sheppard
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Endocrinology     Volume:  100     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1990-01-17     Completed Date:  1990-01-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  629-34     Citation Subset:  AIM; IM    
Department of Cell Biology, Roche Research Center, Hoffmann-La Roche, Inc., Nutley, New Jersey 07110.
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MeSH Terms
Bone and Bones / metabolism*
Calcium / metabolism*
Culture Techniques
Hydroxycholecalciferols / pharmacology*
Structure-Activity Relationship
Reg. No./Substance:
0/Hydroxycholecalciferols; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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