| Effect of hydrogen peroxide on persistent sodium current in guinea pig ventricular myocytes. | |
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MedLine Citation:
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PMID: 15960889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To study the effect of hydrogen peroxide (H2O2) on persistent sodium current (I(Na.P)) in guinea pig ventricular myocytes. METHODS: The whole-cell, cell-attached, and inside-out patch-clamp techniques were applied on isolated ventricular myocytes from guinea pig. RESULTS: H2O2 (0.1 mmol/L, 0.5 mmol/L and 1.0 mmol/L) increased the amplitude of whole-cell I(Na.P) in a concentration-dependent manner, and glutathione (GSH 1 mmol/L) reversed the increased I(Na.P). H2O2 (1 mmol/L) increased persistent sodium channel activity in cell-attached and inside-out patches. The mean open probability was increased from control values of 0.015+/-0.004 and 0.012+/-0.003 to 0.106+/-0.011 and 0.136+/-0.010, respectively (P< 0.01 vs control). They were then decreased to 0.039+/-0.024 and 0.027+/-0.006, respectively, after the addition of 1 mmol/L GSH (P<0.01 vs H2O2). The time when open probability began to increase and reached a maximum was shorter in inside-out patches than that in cell-attached patches (4.8+/-1.0 min vs 11.5+/-3.9 min, P<0.01; 9.6+/-1.6 min vs 18.7+/-4.7 min, P<0.01). CONCLUSION: H2O2 increased the I(Na.P) of guinea pig ventricular myocytes in a concentration-dependent manner, possibly by directly oxidating the cell membrane. |
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Authors:
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Ji-Hua Ma; An-Tao Luo; Pei-Hua Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 26 ISSN: 1671-4083 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-06-17 Completed Date: 2006-08-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: China |
Other Details:
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Languages: eng Pagination: 828-34 Citation Subset: IM |
Affiliation:
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Cardio-Electrophysiological Research Laboratory, Medical College, Wuhan University of Science and Technology, Wuhan 430080, China. mjhua@wust.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dose-Response Relationship, Drug Female Glutathione / pharmacology Guinea Pigs Heart Ventricles Hydrogen Peroxide / administration & dosage, pharmacology* Lidocaine / pharmacology Male Myocytes, Cardiac / physiology* Sodium Channels / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Sodium Channels; 137-58-6/Lidocaine; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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